EXPRESSION OF THE BNMNAP SUBFAMILY OF NAPIN GENES COINCIDES WITH THE INDUCTION OF BRASSICA MICROSPORE EMBRYOGENESIS

被引:75
作者
BOUTILIER, KA
GINES, MJ
DEMOOR, JM
HUANG, B
BASZCZYNSKI, CL
IYER, VN
MIKI, BL
机构
[1] UNIV OTTAWA, DEPT BIOL, OTTAWA, ON K1N 6N5, CANADA
[2] CARLETON UNIV, DEPT BIOL, OTTAWA, ON K1S 5B6, CANADA
[3] PIONEER HI BRED INT INC, DIV PLANT BREEDING, GEORGETOWN, ON L7G 4S7, CANADA
[4] PIONEER HI BRED INT INC, DEPT BIOTECHNOL RES, DIV PLANT BREEDING, JOHNSTON, IA 50131 USA
关键词
BRASSICA NAPUS; MICROSPORE EMBRYOGENESIS; NAPIN;
D O I
10.1007/BF00019486
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Brassica napus cv. Topas microspores can be diverted from pollen development toward haploid embryo formation in culture by subjecting them to a heat stress treatment. We show that this switch in developmental pathways is accompanied by the induction of high levels of napin seed storage protein gene expression. Changes in the plant growth or microspore culture conditions were not by themselves sufficient to induce napin gene expression. Specific members of the napin multigene family were cloned from a cDNA library prepared from microspores that had been induced to undergo embryogenesis. The majority of napin clones represented three members (BnmNAP2, BnmNAP3 and BnmNAP4) that, along with a previously isolated napin genomic clone (BngNAP1), constitute the highly conserved BnmNAP subfamily of napin genes. Both RNA gel blot analysis, using a subfamily-specific probe, and histochemical analysis of transgenic plants expressing a BngNAP1 promoter-beta-glucuronidase gene fusion demonstrated that the BnmNAP subfamily is expressed in embryogenic microspores as well as during subsequent stages of microsporic embryo development.
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页码:1711 / 1723
页数:13
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