FLOW-INJECTION IMMUNOASSAY USING A PROTEIN-A IMMUNOREACTOR

被引：16

作者：

MILLER, JN

PALMER, DA

FRENCH, MT

机构：

[1] Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, Ashby Road

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1991年 / 9卷 / 10-12期

关键词：

CYCLOSPORINE-A; THEOPHYLLINE; FLOW INJECTION IMMUNOASSAY; PROTEIN-A; FLUORESCENCE DETECTION; ELECTROCHEMICAL DETECTION;

D O I：

10.1016/0731-7085(91)80052-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Competitive immunoassays have been developed for the immunosuppressant cyclosporin A and the anti-asthmatic drug theophylline utilizing identical controlled pore glass-protein A microcolumns and flow injection techniques. For cyclosporin A the assay was based on a monoclonal antibody with fluorescence detection whilst for theophylline sheep antiserum and electrochemical detection was used.

引用

页码：1115 / 1120

页数：6

共 30 条

[1]

Bayer E A, 1980, Methods Biochem Anal, V26, P1

[2] USE OF ENZYMES IN IMMUNOASSAY TECHNIQUES - A REVIEW [J].