FLOW-INJECTION IMMUNOASSAY USING A PROTEIN-A IMMUNOREACTOR

被引:16
作者
MILLER, JN
PALMER, DA
FRENCH, MT
机构
[1] Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, Ashby Road
关键词
CYCLOSPORINE-A; THEOPHYLLINE; FLOW INJECTION IMMUNOASSAY; PROTEIN-A; FLUORESCENCE DETECTION; ELECTROCHEMICAL DETECTION;
D O I
10.1016/0731-7085(91)80052-B
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Competitive immunoassays have been developed for the immunosuppressant cyclosporin A and the anti-asthmatic drug theophylline utilizing identical controlled pore glass-protein A microcolumns and flow injection techniques. For cyclosporin A the assay was based on a monoclonal antibody with fluorescence detection whilst for theophylline sheep antiserum and electrochemical detection was used.
引用
收藏
页码:1115 / 1120
页数:6
相关论文
共 30 条
[11]   PROTEIN A FROM STAPHYLOCOCCUS-AUREUS - ITS ISOLATION BY AFFINITY CHROMATOGRAPHY AND ITS USE AS AN IMMUNOSORBENT FOR ISOLATION OF IMMUNOGLOBULINS [J].
HJELM, H ;
HJELM, K ;
SJOQUIST, J .
FEBS LETTERS, 1972, 28 (01) :73-&
[12]   DUAL-COLUMN IMMUNOASSAYS USING PROTEIN-G AFFINITY-CHROMATOGRAPHY [J].
JANIS, LJ ;
REGNIER, FE .
ANALYTICAL CHEMISTRY, 1989, 61 (17) :1901-1906
[14]  
LEE IH, 1988, MIKROCHIM ACTA, V3, P207
[15]   RAPID FLOW-INJECTION SANDWICH-TYPE IMMUNOASSAYS OF PROTEINS USING AN IMMOBILIZED ANTIBODY REACTOR AND ADENOSINE-DEAMINASE ANTIBODY CONJUGATES [J].
LEE, IH ;
MEYERHOFF, ME .
ANALYTICA CHIMICA ACTA, 1990, 229 (01) :47-55
[16]   LIPOSOME FLOW-INJECTION IMMUNOASSAY - IMPLICATIONS FOR SENSITIVITY, DYNAMIC-RANGE, AND ANTIBODY REGENERATION [J].
LOCASCIOBROWN, L ;
PLANT, AL ;
HORVATH, V ;
DURST, RA .
ANALYTICAL CHEMISTRY, 1990, 62 (23) :2587-2593
[17]   COMPETITIVE-BINDING ASSAYS FOR RIBOFLAVIN AND RIBOFLAVIN-BINDING PROTEIN [J].
LOTTER, SE ;
MILLER, MS ;
BRUCH, RC ;
WHITE, HB .
ANALYTICAL BIOCHEMISTRY, 1982, 125 (01) :110-117
[18]  
MATTIASSON B, 1987, ENZYME LABELLED IMMU, P91
[19]   RAPID METHOD FOR THE PURIFICATION OF ANTIBODY-ENZYME CONJUGATES [J].
PAGE, M ;
AUDETTE, M ;
CARON, M .
CANADIAN JOURNAL OF BIOCHEMISTRY, 1979, 57 (03) :286-288
[20]   PROTEIN A-COATED GLASS-BEADS - UNIVERSAL SUPPORT MEDIUM FOR HIGH-PERFORMANCE IMMUNOAFFINITY CHROMATOGRAPHY [J].
PHILLIPS, TM ;
QUEEN, WD ;
MORE, NS ;
THOMPSON, AM .
JOURNAL OF CHROMATOGRAPHY, 1985, 327 (JUN) :213-219