AUTOANTIBODIES AGAINST A SERINE TRANSFER-RNA PROTEIN COMPLEX IMPLICATED IN COTRANSLATIONAL SELENOCYSTEINE INSERTION

被引：120

作者：

GELPI, C ^{[1
]}

SONTHEIMER, EJ ^{[1
]}

RODRIGUEZSANCHEZ, JL ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,HOWARD HUGHES MED INST,DEPT MOLEC BIOPHYS & BIOCHEM,NEW HAVEN,CT 06510

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 20期

关键词：

UGA SUPPRESSOR SERINE TRANSFER RNA THAT CARRIES SELENOCYSTEINE; AUTOIMMUNE CHRONIC ACTIVE HEPATITIS;

D O I：

10.1073/pnas.89.20.9739

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe an autoantibody specificity present in a subgroup of patients with a severe form of autoimmune chronic active hepatitis. These antibodies precipitate a 90-nucleotide RNA from human whole cell extracts and recognize a 48-kDa polypeptide in immunoblotting assays. The RNA is a UGA suppressor serine tRNA that carries selenocysteine (tRNA[Ser]Sec), as shown by sequence analysis. The protein does not appear to be seryl-tRNA synthetase; rather, it is an excellent candidate for a factor involved in cotranslational selenocysteine incorporation in human cells.

引用

页码：9739 / 9743

页数：5

共 40 条

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