SYNTHETIC PEPTIDES OF THE RAB3-EFFECTOR DOMAIN STIMULATE A MEMBRANE-FUSION EVENT INVOLVED IN REGULATED EXOCYTOSIS

被引：35

作者：

EDWARDSON, JM ^{[1
]}

MACLEAN, CM ^{[1
]}

LAW, GJ ^{[1
]}

机构：

[1] AFRC,INST ANIM PHYSIOL & GENET RES,CAMBRIDGE CB2 4AT,ENGLAND

来源：

FEBS LETTERS | 1993年 / 320卷 / 01期

基金：

英国惠康基金;

关键词：

RAB3; GTP-BINDING PROTEIN; MEMBRANE FUSION; EXOCYTOSIS;

D O I：

10.1016/0014-5793(93)81656-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a system in which the fusion of pancreatic zymogen granules with plasma membranes can be studied in vitro. Here we show that this membrane fusion event is stimulated specifically by peptides of the effector domain of rab3, a small, monomeric GTP-binding protein. In addition, we demonstrate that the stimulatory effect of the peptides involves their binding to a target on the plasma membrane, and is both qualitatively and quantitatively different from the effect of GTPgammaS, which also enhances membrane fusion. We suggest that regulated exocytosis in the pancreatic acinar cell may be under the control of more than one type of GTP-binding protein.

引用

页码：52 / 56

页数：5

共 23 条

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