ISOLATION AND PARTIAL CHARACTERIZATION OF MANGANIN, A NEW MANGANOPROTEIN FROM PEANUT SEEDS

Manganin, a new manganoprotein, has been isolated from the seeds of Virginia-type peanuts. The preparation was homogeneous by ultracentrifugal analysis and cellulose polyacetate strip electrophoresis. A minimum molecular weight of 56,300 for manganin was found based on the Mn content. The molecular weight, obtained using the methods of ultracentrifugal analysis of Yphantis and Ehrenberg, was found to be 57,900 and 56,800, respectively. The close correlation between the minimum molecular weight based on the Mn content and the molecular weights obtained from ultracentrifugal analysis indicates that manganin contains one atom of Mn per molecule. The amino acid composition of manganin is distinctly different from that reported for arachin and conarachin, the major globulins of peanut seeds. © 1969.