HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY STUDIES ON PROTEIN - MULTIPLE FORMS OF SOYBEAN LIPOXYGENASE-1

被引：5

作者：

AOSHIMA, H

机构：

[1] Department of Chemistry, Faculty of Liberal Arts, Yamaguchi University, Yoshida Yamaguchi

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90741-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The multiplicity of components of soybean lipoxygenase-1 (EC 1.13.11.12) was shown by high performance liquid chromatography on the cation-exchange resin, TSK Gel LS-212 eluted with sodium phosphate at pH 6.8. Each component of the enzyme also showed a different chromatogram on the TSK Gel W type column, with which the separation is determined mainly by molecular size. However, the enzymes separated by chromatography on TSK Gel LS-212 have similar characteristics (pH optimum, heat stability, and inactivation by H2O2) and similar amino acid composition. The merits and limitations of high performance liquid chromatography in its application to analysis of proteins are discussed. © 1979.

引用

页码：371 / 376

页数：6

共 17 条

[1] AOSHIMA H, 1977, BIOCHIM BIOPHYS ACTA, V486, P121
[2] AOSHIMA H, 1977, INT J PEPT PROT RES, V10, P219
[3] AOSHIMA H, 1977, J BIOCHEM, V82, P569
[4] ISOLATION OF AN ISOZYME OF SOYBEAN LIPOXYGENASE
CHRISTOP.J
PISTORIU.E
AXELROD, B
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 198 (01) : 12 - &
[5] ISOLATION OF A THIRD ISOENZYME OF SOYBEAN LIPOXYGENASE
CHRISTOP.JP
AXELROD, B
PISTORIU.EK
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 284 (01) : 54 - &
[6] DEMONSTRATION BY EPR SPECTROSCOPY OF FUNCTIONAL ROLE OF IRON IN SOYBEAN LIPOXYGENASE-1
DEGROOT, JJM
VELDINK, GA
VLIEGENTHART, JFG
BOLDINGH, J
WEVER, R
VANGELDER, BF
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 377 (01) : 71 - 79
[7] INTERACTION OF SOYBEAN LIPOXYGENASE-1 AND 13-L-HYDROPEROXYLINOLEIC ACID, INVOLVING YELLOW AND PURPLE COLORED ENZYME SPECIES
DEGROOT, JJM
GARSSEN, GJ
VELDINK, GA
VLIEGENTHART, JFG
BOLDINGH, J
EGMOND, MR
[J]. FEBS LETTERS, 1975, 56 (01) : 50 - 54
[8] CHANGES IN FLUORESCENCE AND ABSORBANCE OF LIPOXYGENASE-1 INDUCED BY 13-LS-HYDROPEROXYLINOLEIC ACID AND LINOLEIC-ACID
EGMOND, MR
FINAZZIAGRO, A
FASELLA, PM
VELDINK, GA
VLIEGENTHART, JFG
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 397 (01) : 43 - 49
[9] HATANO H, 1977, HIGH SPEED LIQUID CH, V3
[10] INACTIVATION OF LIPOXYGENASE BY HYDROGEN PEROXIDE CYSTEINE AND SOME OTHER REAGENTS
MITSUDA, H
YASUMOTO, K
YAMAMOTO, A
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1967, 31 (07): : 853 - &

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