PRIOR USE OF THE NEUTRAL RED ASSAY AND REDUCTION OF TOTAL PROTEIN DETERMINATION IN 96-WELL PLATE ASSAYS

The sequential use of the neutral red and protein assays in 96-well plates leads to approximately a 50% underestimation of the amount of protein. In absolute terms, more protein is lost from wells having more protein, but within a cell type the reduction appears to be proportional to the amount of protein. This should be taken into account when interpreting data obtained in this way. © 1990.