CLONING, NUCLEOTIDE SEQUENCING, AND EXPRESSION IN ESCHERICHIA-COLI OF A LIPASE AND ITS ACTIVATOR GENES FROM PSEUDOMONAS SP KWI-56

被引：61

作者：

IIZUMI, T ^{[1
]}

NAKAMURA, K ^{[1
]}

SHIMADA, Y ^{[1
]}

SUGIHARA, A ^{[1
]}

TOMINAGA, Y ^{[1
]}

FUKASE, T ^{[1
]}

机构：

[1] OSAKA MUNICIPAL TECH RES INST,JOTO KU,OSAKA 536,JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1991年 / 55卷 / 09期

关键词：

D O I：

10.1080/00021369.1991.10870953

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A lipase gene (lip) and its activator gene (act) on a 2.9 kb BglII-EcoRI fragment from Pseudomonas sp. KWI-56 were cloned in Escherichia coli using pUC19 as a vector plasmid. From the sequencing results, the open reading frames of the lip and the act were found to contain 1092 and 1032 nucleotides, respectively. The act existed downstream of the lip with the same orientation. When the lip was expressed in E. coli using the lac promoter on the pUC plasmid vector, the lipase activity of E. coli carrying both the lip and the act was 200-fold greater than that carrying only the lip. This result suggested the act was important in the expression of the lip in E. coli.

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页码：2349 / 2357

页数：9

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