CALBINDIN D-27 KDA - PREFERENTIAL LOCALIZATION IN NON-B ISLET CELLS OF THE RAT PANCREAS

The presence and abundance of calbindin in rat pancreatic islet cells was assessed by immunohistochemistry of either whole islets or purified B and non-B islet cells as well as by Western blotting of extracts derived from whole islets and purified B and non-B islet cells. Immunohistochemistry of pancreatic sections indicated a higher calbindin content in non-B cells, located at the periphery of the islets, than in the centrally located insulin-producing B cells. Comparable results were obtained in purified islet cells. Likewise, scanning densitometry of the Western blots indicated that, relative to cell volume, the single calbindin band (Mr 27 kDa) was 5-7 times higher in non-B than in B cells. In the splenic lobe of chick pancreas, however, the opposite situation prevailed. Thus, insulin-producing cells clustered in small roundish islets were more intensely labelled after exposure to anti-calbindin serum than non-B islet cells located in large and irregularly shaped islets. Nevertheless, even in the chick pancreas, non-B islet cells contained an appreciable amount of calbindin.