INHIBITION OF MOUSE LYMPHOCYTE PROLIFERATIVE RESPONSE BY GLYCOSPHINGOLIPIDS FROM LEISHMANIA (L) AMAZONENSIS

被引：14

作者：

GIORGIO, S

JASIULIONIS, MG

STRAUS, AH

TAKAHASHI, HK

BARBIERI, CL

机构：

[1] ESCOLA PAULISTA MED SCH,DEPT MICROBIOL IMMUNOL & PARASITOL,RUA BOTUCATU 862,6 ANDAR,BR-04023 SAO PAULO,BRAZIL

[2] ESCOLA PAULISTA MED SCH,DEPT BIOCHEM,BR-04023 SAO PAULO,BRAZIL

来源：

EXPERIMENTAL PARASITOLOGY | 1992年 / 75卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

LEISHMANIA (L) AMAZONENSIS; LYMPHOCYTES; LEISHMANIASIS;

D O I：

10.1016/0014-4894(92)90127-V

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The effect of a purified preparation of glycosphingolipids (GSLs) extracted from Leishmania (L.) amazonensis amastigotes on murine lymphocytes was investigated. GSLs inhibited both concanavalin A (Con A)- or lipopolisaccharide-induced [3H]thymidine uptake by normal and immunized BALB/c mouse lymph node cells. The effect of total GSLs was dose dependent. Total GSLs also suppressed the two-way mixed lymphocyte reaction of BALB/c × C57BL/6 cells and the antigen-specific response of immunized mouse cells. Six pure bands as well as a pool containing a mixture of GSLs with five to seven sugar residues separated by a combination of HPLC and preparative HPTLC were tested and shown to be inhibitors of Con A stimulation. These results suggest that parasite glycosphingolipids may play an immunologically relevant role in leishmaniasis. © 1992.

引用

页码：119 / 125

页数：7

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