THE HISTOPATHOLOGICAL, BEHAVIORAL AND NEUROCHEMICAL EFFECTS OF INTRAVENTRICULAR-INJECTION OF ETHYLCHOLINE MUSTARD AZIRIDINIUM (AF64A) IN THE NEONATAL RAT

被引：9

作者：

ARMSTRONG, JN ^{[1
]}

PAPPAS, BA ^{[1
]}

机构：

[1] CARLETON UNIV,DEPT PSYCHOL,OTTAWA K1S 5B6,ONTARIO,CANADA

来源：

DEVELOPMENTAL BRAIN RESEARCH | 1991年 / 61卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CHOLINERGIC NEUROTOXIN; ETHYLCHOLINE AZIRIDINIUM; HIPPOCAMPUS; SPATIAL MEMORY;

D O I：

10.1016/0165-3806(91)90138-9

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study investigated the histopathological, behavioral and neurochemical effects of bilateral injection of 2.0, 0.5 and 0.1 nmol/ventricle ethylcholine aziridinium (AF64A) on postnatal day (PND) 2. The rats showed a significant, but non-dose-related reduction of choline acetyltransferase (ChAT) in the hippocampus but not the cerebral cortex or the caudate nucleus when sacrificed on PND 16. No effect on ChAT was found in any region at PND 58. The group given 2 nmol/ventricle were hyperactive and showed a deficit in spatial learning when tested on the Morris water maze at PND 38-43. No such differences were observed for the rats injected with 0.1 or 0.5 nmol/ventricle AF64A. This spatial learning impairment in the 2 nmol group was associated with non-specific tissue damage seen only in animals from this group that were sacrificed at PND 40. This tissue damage was most evident in the left medial frontal cortex, the caudate nuclei and the anterior dorsal hippocampus.

引用

页码：249 / 257

页数：9

共 33 条

[21] IS ETHYLCHOLINE MUSTARD AZIRIDINIUM ION A SPECIFIC CHOLINERGIC NEUROTOXIN [J].

MCGURK, SR ;

HARTGRAVES, SL ;

KELLY, PH ;

GORDON, MN ;

BUTCHER, LL .

NEUROSCIENCE, 1987, 22 (01) :215-224

[22] PLACE NAVIGATION IMPAIRED IN RATS WITH HIPPOCAMPAL-LESIONS [J].

MORRIS, RGM ;

GARRUD, P ;

RAWLINS, JNP ;

OKEEFE, J .

NATURE, 1982, 297 (5868) :681-683

[23] NEONATAL AND ADULT FOREBRAIN NOREPINEPHRINE DEPLETION AND THE BEHAVIORAL AND CORTICAL THICKENING EFFECTS OF ENRICHED IMPOVERISHED ENVIRONMENT [J].

MURTHA, S ;

PAPPAS, BA ;

RAMAN, S .

BEHAVIOURAL BRAIN RESEARCH, 1990, 39 (03) :249-261

[24]

OKeefe J., 1978, HIPPOCAMPUS COGNITIV

[25]

Olds M.E., 1961, BRAIN MECHANISMS LEA, P153

[26] HIPPOCAMPAL CONNECTIONS AND SPATIAL DISCRIMINATION [J].

OLTON, DS ;

WALKER, JA ;

GAGE, FH .

BRAIN RESEARCH, 1978, 139 (02) :295-308

[27]

PAXINOS G, 1986, AT BRAIN STETEOTAXIC

[28] VALIDATION OF OPEN - CLOSED ARM ENTRIES IN AN ELEVATED PLUS-MAZE AS A MEASURE OF ANXIETY IN THE RAT [J].

PELLOW, S ;

CHOPIN, P ;

FILE, SE ;

BRILEY, M .

JOURNAL OF NEUROSCIENCE METHODS, 1985, 14 (03) :149-167

[29]

PLACTEAU C, 1989, BEHAV BRAIN RES, V34, P79

[30] INHIBITION OF HIGH-AFFINITY CHOLINE TRANSPORT ATTENUATES BOTH CHOLINERGIC AND NON-CHOLINERGIC EFFECTS OF ETHYLCHOLINE AZIRIDINIUM (AF64A) [J].

POTTER, PE ;

TEDFORD, CE ;

KINDEL, G ;

HANIN, I .

BRAIN RESEARCH, 1989, 487 (02) :238-244

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