CALCIUM INFLUX MEDIATED BY THE ESCHERICHIA-COLI HEAT-STABLE ENTEROTOXIN-B (ST(B))

被引:72
作者
DREYFUS, LA [1 ]
HARVILLE, B [1 ]
HOWARD, DE [1 ]
SHABAN, R [1 ]
BEATTY, DM [1 ]
MORRIS, SJ [1 ]
机构
[1] UNIV MISSOURI, DIV MOLEC BIOL & BIOCHEM, KANSAS CITY, MO 64110 USA
关键词
D O I
10.1073/pnas.90.8.3202
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The heat-stable enterotoxin B (ST(B)) of Escherichia coli is a 48-amino acid extracellular peptide that induces rapid fluid accumulation in animal intestinal models. Unlike other E. coli enterotoxins that elicit cAMP or cGMP responses in the gut [heat-labile toxin (LT) and heat-stable toxin A (ST(A)), respectively], ST(B) induces fluid loss by an undefined mechanism that is independent of cyclic nucleotide elevation. Here we studied the effects of ST(B) on intracellular calcium concentration ([Ca2+]i), another known mediator of intestinal ion and fluid movement. Ca2+ and pH measurements were performed on different cell types including Madin-Darby canine kidney (MDCK), HT-29/C1 intestinal epithelial cells, and primary rat pituitary cells, Ca2+ and pH determinations were performed by simultaneous real-time fluorescence imaging at four emission wavelengths. This allowed dual imaging of the Ca2+- and pH-specific ratio dyes (indo-1 and SNARF-1, respectively). ST(B) treatment induced a dose-dependent increase in [Ca2+]i with virtually no effect on internal pH in all of the cell types tested. ST(B)-mediated [Ca2+]i elevation was not inhibited by drugs that block voltage-gated Ca2+ channels including nitrendipine, verapamil (L-type), omega-conotoxin (N-type), and Ni2+ (T-type). The increase in [Ca2+]i was dependent on a source of extracellular Ca2+ and was not affected by prior treatment of MDCK cells with thapsigargin or cyclopiazonic acid, agents that deplete and block internal Ca2+ stores. In contrast to these results, somatostatin and pertussis toxin pretreatment of MDCK cells completely blocked the ST(B)-induced increase in [Ca2+]i. Taken together, these data suggest that ST(B) opens a GTP-binding regulatory protein-linked receptor-operated Ca2+ channel in the plasma membrane. The nature of the ST(B)-sensitive Ca2+ channel is presently under investigation.
引用
收藏
页码:3202 / 3206
页数:5
相关论文
共 41 条
[1]  
ABDELLATIF AA, 1986, PHARMACOL REV, V38, P227
[2]   NITRENDIPINE BLOCK OF CARDIAC CALCIUM CHANNELS - HIGH-AFFINITY BINDING TO THE INACTIVATED STATE [J].
BEAN, BP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (20) :6388-6392
[3]   RECEPTOR-EFFECTOR COUPLING BY G-PROTEINS [J].
BIRNBAUMER, L ;
ABRAMOWITZ, J ;
BROWN, AM .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1031 (02) :163-224
[4]   BIOLOGICAL EVALUATION OF A METHANOL-SOLUBLE, HEAT-STABLE ESCHERICHIA-COLI ENTEROTOXIN IN INFANT MICE, PIGS, RABBITS, AND CALVES [J].
BURGESS, MN ;
BYWATER, RJ ;
COWLEY, CM ;
MULLAN, NA ;
NEWSOME, PM .
INFECTION AND IMMUNITY, 1978, 21 (02) :526-531
[5]   SIGNAL TRANSDUCTION BY GUANYLYL CYCLASES [J].
CHINKERS, M ;
GARBERS, DL .
ANNUAL REVIEW OF BIOCHEMISTRY, 1991, 60 :553-575
[6]  
Chronwall B M, 1988, Peptides, V9 Suppl 1, P169, DOI 10.1016/0196-9781(88)90241-0
[7]   TEST FOR ESCHERICHIA-COLI ENTEROTOXIN USING INFANT MICE - APPLICATION IN A STUDY OF DIARRHEA IN CHILDREN IN HONOLULU [J].
DEAN, AG ;
WILLIAMS, RG ;
CHING, Y ;
HARDEN, LB .
JOURNAL OF INFECTIOUS DISEASES, 1972, 125 (04) :407-&
[8]   PURIFICATION OF THE STB ENTEROTOXIN OF ESCHERICHIA-COLI AND THE ROLE OF SELECTED AMINO-ACIDS ON ITS SECRETION, STABILITY AND TOXICITY [J].
DREFUS, LA ;
URBAN, RG ;
WHIPP, SC ;
SLAUGHTER, C ;
TACHIAS, K ;
KUPERSZTOCH, YM .
MOLECULAR MICROBIOLOGY, 1992, 6 (16) :2397-2406
[9]   CHEMICAL-PROPERTIES OF HEAT-STABLE ENTEROTOXINS PRODUCED BY ENTERO-TOXIGENIC ESCHERICHIA-COLI OF DIFFERENT HOST ORIGINS [J].
DREYFUS, LA ;
FRANTZ, JC ;
ROBERTSON, DC .
INFECTION AND IMMUNITY, 1983, 42 (02) :539-548
[10]   HEAT-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI - INVITRO EFFECTS ON GUANYLATE CYCLASE ACTIVITY, CYCLIC-GMP CONCENTRATION, AND ION-TRANSPORT IN SMALL-INTESTINE [J].
FIELD, M ;
GRAF, LH ;
LAIRD, WJ ;
SMITH, PL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (06) :2800-2804