MOLECULAR-GENETICS OF THE GLUTAMINE SYNTHETASES IN RHIZOBIUM SPECIES

Soil bacteria of the genus Rhizobium and Bradyrhizobium establish symbiotic interactions with leguminous plants that result in the formation of specialized structures, the nodules, in which the bacteria differentiate into bacteroids and fix nitrogen. Rhizobial glutamine synthetase (GS) activity is very low in the nodule.(1) The ammonia produced by the bacteroids is exported to the plant cell, where it is assimilated by the GS from the plant,(2,3) whereas in the free-living state, Rhizobium and Bradyrhizobium species assimilate ammonia for growth. Another characteristic of these species is that they possess two glutamine synthetase isozymes, known as GSI and GSII.(4,5) A third glutamine synthetase isozyme, called GSIII, has been found in R. meliloti(6,7) and R. etli.(8,9)