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SPECTRAL CHARACTERIZATION OF NEW CHLOROPHYLL-C PIGMENTS ISOLATED FROM EMILIANIA-HUXLEYI (PRYMNESIOPHYCEAE) BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
被引:25
作者:
GARRIDO, JL
[1
]
ZAPATA, M
[1
]
MUNIZ, S
[1
]
机构:
[1] CONSELLERIA PESCA XUNTA DE GALICIA,CTR INVEST MARINAS,E-36600 VILAGARCIA AROUSA,SPAIN
关键词:
CHLOROPHYLL C PIGMENTS;
CHLOROPHYLL C(3);
EMILIANIA HUXLEYI;
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;
MAGNESIUM-3,8-DIVINYLPHEOPORPHYRIN A(5) MONOMETHYL ESTER;
PELAGOCOCCUS SUBVIRIDIS;
PHYTOL-SUBSTITUTED;
D O I:
10.1111/j.0022-3646.1995.00761.x
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
Pigment extracts from the prymnesiophyte Emiliania huxleyi (Lohm.) Hay & Mohler (CCMP 370) were analyzed using a new chromatographic method based on the use of polymeric octadecylsilica columns, which split both the chlorophyll c(3) peak and phytol-substituted chlorophyll c-like peak into two fractions. The pigments corresponding to each of these fractions were isolated. The spectra and chromatographic behavior suggest that chlorophyll c(3) from E. huxleyi is a mixture of two different compounds whereas the splitting of the phytol-substitute c-like pigment could be the result of the equilibrium of two forms showing the same absorption spectrum. The method allowed, as well, the detection of magnesium 3,8-divinylpheoporphyrin a(5) monomethyl ester in the prymnesiophytes E. huxleyi (CCMP 370) and Prymnesium parvum Carter (CCMP 708) and the chrysophyte Pelagococcus subviridis Norris (CCMP 1429).
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页码:761 / 768
页数:8
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