ANNULAR APERTURE 2-PHOTON EXCITATION MICROSCOPY

被引：58

作者：

HELL, SW ^{[1
]}

HANNINEN, PE ^{[1
]}

KUUSISTO, A ^{[1
]}

SCHRADER, M ^{[1
]}

SOINI, E ^{[1
]}

机构：

[1] CTR BIOTECHNOL,SF-20521 TURKU,FINLAND

来源：

OPTICS COMMUNICATIONS | 1995年 / 117卷 / 1-2期

关键词：

D O I：

10.1016/0030-4018(95)00115-O

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We investigate the effect of annular apertures in two-photon excitation confocal and non-confocal microscopy. In a theoretical study, we show that the use of annular apertures in two-photon excitation microscopy leads to resolution increase without diffraction fringes. We determine experimentally the axial resolution of annular aperture confocal and non-confocal two-photon excitation microscopes and compare it with that of the standard two-photon excitation contrasts. Images of fluorescence beads obtained with a transmission annular aperture two-photon excitation microscope demonstrate the applicability of annular apertures for enlarging the focal depth in high resolution two-photon excitation imaging.

引用

页码：20 / 24

页数：5

共 16 条

[1]

ARIMOTO R, 1990, OPTIK, V86, P7

[2] CONFOCAL SCANNING LIGHT-MICROSCOPY WITH HIGH APERTURE IMMERSION LENSES [J].