IMPROVED AXIAL RESOLUTION IN CONFOCAL FLUORESCENCE MICROSCOPY USING ANNULAR PUPILS

被引:26
作者
GU, M
TANNOUS, T
SHEPPARD, CJR
机构
[1] Department of Physical Optics, University of Sydney, Sydney
关键词
D O I
10.1016/0030-4018(94)90245-3
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
The axial image of a thick fluorescent layer is studied in a confocal microscope consisting of either circular or annular pupils. The response for circular pupils is sharper than that for annular pupils if a small pinhole detector is used. But when the size of the pinhole is larger than a certain value, the response in the latter case can become sharper than that in the former. This result implies that for a given detector of finite size, axial resolution in confocal fluorescence microscopy can be improved when an annular lens is used. Our experimental results qualitatively demonstrate the theoretical prediction. The strength of optical sectioning and the axial cross-section of the three-dimensional optical transfer function are also derived from the measured data.
引用
收藏
页码:533 / 539
页数:7
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