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THERMODYNAMIC PROFILES OF PENICILLIN-G HYDROLYSIS CATALYZED BY WILD-TYPE AND MET-]ALA168 MUTANT PENICILLIN ACYLASES FROM KLUYVERA-CITROPHILA
被引:24
作者
:
MARTIN, J
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV COMPLUTENSE MADRID, FAC QUIM, DEPT BIOQUIM & BIOL MOLEC 1, E-28040 MADRID, SPAIN
MARTIN, J
PRIETO, I
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV COMPLUTENSE MADRID, FAC QUIM, DEPT BIOQUIM & BIOL MOLEC 1, E-28040 MADRID, SPAIN
PRIETO, I
BARBERO, JL
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV COMPLUTENSE MADRID, FAC QUIM, DEPT BIOQUIM & BIOL MOLEC 1, E-28040 MADRID, SPAIN
BARBERO, JL
PEREZGIL, J
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV COMPLUTENSE MADRID, FAC QUIM, DEPT BIOQUIM & BIOL MOLEC 1, E-28040 MADRID, SPAIN
PEREZGIL, J
MANCHENO, JM
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV COMPLUTENSE MADRID, FAC QUIM, DEPT BIOQUIM & BIOL MOLEC 1, E-28040 MADRID, SPAIN
MANCHENO, JM
ARCHE, R
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV COMPLUTENSE MADRID, FAC QUIM, DEPT BIOQUIM & BIOL MOLEC 1, E-28040 MADRID, SPAIN
ARCHE, R
机构
:
[1]
UNIV COMPLUTENSE MADRID, FAC QUIM, DEPT BIOQUIM & BIOL MOLEC 1, E-28040 MADRID, SPAIN
[2]
ANTIBIOT FARMA SA, DEPT GENET MOLEC, MADRID, SPAIN
来源
:
BIOCHIMICA ET BIOPHYSICA ACTA
|
1990年
/ 1037卷
/ 02期
关键词
:
Penicillin acylase;
Reaction thermodynamics;
Site directed mutagenesis;
D O I
:
10.1016/0167-4838(90)90158-C
中图分类号
:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号
:
071010 ;
081704 ;
摘要
:
The Met-168 residue in penicillin acylase from Kluyvera citrophila was changed to Ala by oligonucleotide site-directed mutagenesis. The Ala-168 mutant exhibited different substrate specificity than wild-type and enhanced thermal stability. The thermodynamic profiles for penicillin G hydrolysis catalyzed by both enzymes were obtained from the temperature dependence of the steady-state kinetic parameters Km and kcat. The high values of enthalpy and entropy of activation determined for the binding of substrate suggest that an induced-fit-like mechanism takes place. The Met → Ala168 mutation unstabilizes the first transition-state (E··S≠) and the enzyme-substrate complex (ES) causing a decrease in association equilibrium and specificity constants in the enzyme. However, no change is observed in the acyl-enzyme formation. It is concluded that residue 168 is involved in the enzyme conformational rearrangements caused by the interaction of the acid moiety of the substrate at the active site. © 1990.
引用
收藏
页码:133 / 139
页数:7
相关论文
共 34 条
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THE MECHANISM OF IRREVERSIBLE ENZYME INACTIVATION AT 100-DEGREES-C
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KLIBANOV, AM
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APPLICATION OF THE FLUORESCAMINE REACTION WITH 6-AMINOPENICILLANIC ACID TO ESTIMATION AND DETECTION OF PENICILLIN ACYLASE ACTIVITY
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ISOLATION AND KINETICS OF PENICILLIN AMIDASE FROM ESCHERICHIA-COLI
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BALASINGHAM, K
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0
引用数:
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WARBURTON, D
论文数:
0
引用数:
0
h-index:
0
WARBURTON, D
;
DUNNILL, P
论文数:
0
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0
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DUNNILL, P
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LILLY, MD
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0
LILLY, MD
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COMPLETE NUCLEOTIDE-SEQUENCE OF THE PENICILLIN ACYLASE GENE FROM KLUYVERA-CITROPHILA
[J].
BARBERO, JL
论文数:
0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
BARBERO, JL
;
BUESA, JM
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0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
BUESA, JM
;
DEBUITRAGO, GG
论文数:
0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
DEBUITRAGO, GG
;
MENDEZ, E
论文数:
0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
MENDEZ, E
;
PEREZARANDA, A
论文数:
0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
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;
GARCIA, JL
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0
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CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
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DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS
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BEAUCAGE, SL
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引用数:
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h-index:
0
机构:
UNIV COLORADO, DEPT CHEM, BOULDER, CO 80309 USA
UNIV COLORADO, DEPT CHEM, BOULDER, CO 80309 USA
BEAUCAGE, SL
;
CARUTHERS, MH
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV COLORADO, DEPT CHEM, BOULDER, CO 80309 USA
UNIV COLORADO, DEPT CHEM, BOULDER, CO 80309 USA
CARUTHERS, MH
.
TETRAHEDRON LETTERS,
1981,
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STRUCTURAL PLASTICITY BROADENS THE SPECIFICITY OF AN ENGINEERED PROTEASE
[J].
BONE, R
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
BONE, R
;
SILEN, JL
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
SILEN, JL
;
AGARD, DA
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
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FACTORS AFFECTING SYNTHESIS OF AMPICILLIN AND HYDROXYPENICILLINS BY CELL-BOUND PENICILLIN ACYLASE OF ESCHERICHIA COLI
[J].
COLE, M
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0
引用数:
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COLE, M
.
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[9]
HYDROLYSIS OF PENICILLINS AND RELATED COMPOUNDS BY CELL-BOUND PENICILLIN ACYLASE OF ESCHERICHIA COLI
[J].
COLE, M
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0
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0
COLE, M
.
BIOCHEMICAL JOURNAL,
1969,
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DEACYLATION OF ACYLAMINO COMPOUNDS OTHER THAN PENICILLINS BY CELL-BOUND PENICILLIN ACYLASE OF ESCHERICHIA COLI
[J].
COLE, M
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0
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0
COLE, M
.
BIOCHEMICAL JOURNAL,
1969,
115
(04)
:741
-&
←
1
2
3
4
→
共 34 条
[1]
THE MECHANISM OF IRREVERSIBLE ENZYME INACTIVATION AT 100-DEGREES-C
[J].
AHERN, TJ
论文数:
0
引用数:
0
h-index:
0
AHERN, TJ
;
KLIBANOV, AM
论文数:
0
引用数:
0
h-index:
0
KLIBANOV, AM
.
SCIENCE,
1985,
228
(4705)
:1280
-1284
[2]
APPLICATION OF THE FLUORESCAMINE REACTION WITH 6-AMINOPENICILLANIC ACID TO ESTIMATION AND DETECTION OF PENICILLIN ACYLASE ACTIVITY
[J].
BAKER, WL
论文数:
0
引用数:
0
h-index:
0
BAKER, WL
.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,
1983,
23
(01)
:26
-30
[3]
ISOLATION AND KINETICS OF PENICILLIN AMIDASE FROM ESCHERICHIA-COLI
[J].
BALASINGHAM, K
论文数:
0
引用数:
0
h-index:
0
BALASINGHAM, K
;
WARBURTON, D
论文数:
0
引用数:
0
h-index:
0
WARBURTON, D
;
DUNNILL, P
论文数:
0
引用数:
0
h-index:
0
DUNNILL, P
;
LILLY, MD
论文数:
0
引用数:
0
h-index:
0
LILLY, MD
.
BIOCHIMICA ET BIOPHYSICA ACTA,
1972,
276
(01)
:250
-+
[4]
COMPLETE NUCLEOTIDE-SEQUENCE OF THE PENICILLIN ACYLASE GENE FROM KLUYVERA-CITROPHILA
[J].
BARBERO, JL
论文数:
0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
BARBERO, JL
;
BUESA, JM
论文数:
0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
BUESA, JM
;
DEBUITRAGO, GG
论文数:
0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
DEBUITRAGO, GG
;
MENDEZ, E
论文数:
0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
MENDEZ, E
;
PEREZARANDA, A
论文数:
0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
PEREZARANDA, A
;
GARCIA, JL
论文数:
0
引用数:
0
h-index:
0
机构:
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
CTR RAMON & CAJAL, DEPT ENDOCRINOL, E-28034 MADRID, SPAIN
GARCIA, JL
.
GENE,
1986,
49
(01)
:69
-80
[5]
DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS
[J].
BEAUCAGE, SL
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV COLORADO, DEPT CHEM, BOULDER, CO 80309 USA
UNIV COLORADO, DEPT CHEM, BOULDER, CO 80309 USA
BEAUCAGE, SL
;
CARUTHERS, MH
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV COLORADO, DEPT CHEM, BOULDER, CO 80309 USA
UNIV COLORADO, DEPT CHEM, BOULDER, CO 80309 USA
CARUTHERS, MH
.
TETRAHEDRON LETTERS,
1981,
22
(20)
:1859
-1862
[6]
STRUCTURAL PLASTICITY BROADENS THE SPECIFICITY OF AN ENGINEERED PROTEASE
[J].
BONE, R
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
BONE, R
;
SILEN, JL
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
SILEN, JL
;
AGARD, DA
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
AGARD, DA
.
NATURE,
1989,
339
(6221)
:191
-195
[7]
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]
FACTORS AFFECTING SYNTHESIS OF AMPICILLIN AND HYDROXYPENICILLINS BY CELL-BOUND PENICILLIN ACYLASE OF ESCHERICHIA COLI
[J].
COLE, M
论文数:
0
引用数:
0
h-index:
0
COLE, M
.
BIOCHEMICAL JOURNAL,
1969,
115
(04)
:757
-&
[9]
HYDROLYSIS OF PENICILLINS AND RELATED COMPOUNDS BY CELL-BOUND PENICILLIN ACYLASE OF ESCHERICHIA COLI
[J].
COLE, M
论文数:
0
引用数:
0
h-index:
0
COLE, M
.
BIOCHEMICAL JOURNAL,
1969,
115
(04)
:733
-&
[10]
DEACYLATION OF ACYLAMINO COMPOUNDS OTHER THAN PENICILLINS BY CELL-BOUND PENICILLIN ACYLASE OF ESCHERICHIA COLI
[J].
COLE, M
论文数:
0
引用数:
0
h-index:
0
COLE, M
.
BIOCHEMICAL JOURNAL,
1969,
115
(04)
:741
-&
←
1
2
3
4
→