GENETIC-MAPPING OF TOMATO CDNA CLONES ENCODING THE CHLOROPLASTIC AND THE CYTOSOLIC ISOZYMES OF SUPEROXIDE-DISMUTASE

被引:14
作者
PERLTREVES, R [1 ]
ABUABIED, M [1 ]
MAGAL, N [1 ]
GALUN, E [1 ]
ZAMIR, D [1 ]
机构
[1] HEBREW UNIV JERUSALEM,FAC AGR,IL-76100 REHOVOT,ISRAEL
关键词
isozymes; Lycopersicon; restriction fragment length polymorphism (RFLP) map; superoxide dismutase;
D O I
10.1007/BF00554381
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The isozyme pattern of superoxide dismutase (SOD) in tomato consists of two Cu,Zn isozymes located, respectively, in the chloroplast and in the cytosol, as well as additional isozymes of the Mn or Fe SOD type. We have shown that SOD-1 is the chloroplastic Cu,Zn SOD and is related to cDNA clone T10. Restriction fragment length polymorphism (RFLP) analysis was performed with two cDNA clones representing tomato Cu,Zn-superoxide dismutases. T10, coding for the chloroplast isozyme, was thus mapped to chromosome 11, between marker TG46 and TG108, while clone P31, coding for the cytosolic Cu,Zn SOD isozyme, was mapped to chromosome 1 between TG24 and TG81. SOD is associated with the response of plants to various environmental stresses; the mapping information presented here would permit the demonstration of this association by genetic analysis. © 1990 Plenum Publishing Corporation.
引用
收藏
页码:543 / 552
页数:10
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