EFFECTS OF INTERMEDIATE EXCHANGE PROCESSES ON THE ESTIMATION OF EQUILIBRIUM-CONSTANTS BY NMR

被引：108

作者：

FEENEY, J

BATCHELOR, JG

ALBRAND, JP

ROBERTS, GCK

机构：

[1] Division of Molecular Pharmacology, National Institute for Medical Research, London, NW7 1AA, Mill Hill

来源：

JOURNAL OF MAGNETIC RESONANCE | 1979年 / 33卷 / 03期

关键词：

D O I：

10.1016/0022-2364(79)90163-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the analysis of NMR binding curves for ligands binding to macromolecules, if the assumption of fast exchange is not justified there will be errors in the estimated equilibrium constants and the bound chemical shifts. The magnitudes of these systematic errors have been calculated for different sets of parameters and shown to be large, particularly when the lowest concentration of ligand examined is greater than that of the macromolecule. Methods of detecting these errors are discussed. © 1979.

引用

页码：519 / 529

页数：11

共 15 条

[11] NUCLEAR MAGNETIC RESONANCE STUDIES OF SOLUTIONS OF RARE-EARTH IONS AND THEIR COMPLEXES .4. CONCENTRATION AND TEMPERATURE DEPENDENCE OF OXYGEN-14 TRANSVERSE RELAXATION IN AQUEOUS SOLUTIONS [J].