COMPARATIVE GENOMIC HYBRIDIZATION AS A TOOL TO DEFINE 2 DISTINCT CHROMOSOME-12-DERIVED AMPLIFICATION UNITS IN WELL-DIFFERENTIATED LIPOSARCOMAS

被引：61

作者：

SUIJKERBUIJK, RF

WEGHUIS, DEM

VANDENBERG, M

PEDEUTOUR, F

FORUS, A

MYKLEBOST, O

GLIER, C

TURCCAREL, C

VANKESSEL, AG

机构：

[1] LAB GENET MOLEC CANC HUMAINS,CNRS,URA 1462,NICE,FRANCE

[2] BIOL DETECT SYST INC,ROCKVILLE,MD

[3] NORWEGIAN RADIUM HOSP,DEPT TUMOR BIOL,OSLO 3,NORWAY

来源：

GENES CHROMOSOMES & CANCER | 1994年 / 9卷 / 04期

关键词：

D O I：

10.1002/gcc.2870090410

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Well-differentiated liposarcomas (WDLPS) are frequently characterized by a near-diploid karyotype with supernumerary ring and/or giant rod-shaped marker chromosomes. We have shown, using fluorescence in situ hybridization (FISH) and molecular strategies, that these markers contain chromosome 12-derived sequences. Here we report the analysis of six WDLPS for the presence of amplified DNA segments by means of the recently developed comparative genomic hybridization (CGH) strategy. Two distinct chromosome 12-derived amplification units could be identified in all tumors examined, one located in the q14-q15 region as expected, the second unexpectedly mapping to q21.3-q22. Our results indicate that the concerted amplification of these two distinct regions on the long arm of chromosome 12 may be a consistent characteristic of WDLPS. These amplifications are most likely directly related to the presence of supernumerary ring and/or giant marker chromosomes in this group of soft tissue tumors. (C) 1994 Wiley-Liss, Inc.

引用

页码：292 / 295

页数：4

共 17 条

[11]

SCHOENMAKERS HFP, 1993, CYTOGENET CELL GENET, V62, P159

[12]

SMITH SH, 1992, CANCER RES, V52, P3746

[13]

SREEKANTAIAH C, 1992, CANCER, V69, P2484

[14] ANALYSIS OF A GIANT MARKER CHROMOSOME IN A WELL-DIFFERENTIATED LIPOSARCOMA USING CYTOGENETICS AND FLUORESCENCE INSITU HYBRIDIZATION [J].