ENZYMATIC-ACTIVITY OF CELLULASE ADSORBED ON CELLULOSE AND ITS CHANGE DURING HYDROLYSIS

被引：45

作者：

OOSHIMA, H ^{[1
]}

KURAKAKE, M ^{[1
]}

KATO, J ^{[1
]}

HARANO, Y ^{[1
]}

机构：

[1] FUKUYAMA UNIV,DEPT FOOD SCI & TECHNOL,FUKUYAMA,HIROSHIMA 72902,JAPAN

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1991年 / 31卷 / 03期

关键词：

ENZYMATIC HYDROLYSIS OF CELLULOSE; CELLULASE; KINETICS; ADSORPTION; AVICEL;

D O I：

10.1007/BF02921752

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrolysis of pure cellulose Avicel has been carried out, using Meicelase from Trichoderma viride, where the enzymatic activity of cellulase adsorbed on cellulose and its changes during the hydrolysis were investigated. A rapid drop of the hydrolysis rate during the reaction that is always observed in enzymatic hydrolysis of cellulose, could be explained by a decline of specific activity of adsorbed enzyme, and it was implied that the decline results from a loss of synergistic action between endoglucanase and exoglucanase. An empirical equation expresses the change of hydrolysis rate during the reaction and also shows that the change of the hydrolysis rate is caused by the decline of the specific enzymatic activity of adsorbed enzyme.

引用

页码：253 / 266

页数：14

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