CHARACTERIZATION OF THE EFFECTOR MECHANISMS OF A TRANSMISSION-BLOCKING ANTIBODY UPON DIFFERENTIATION OF PLASMODIUM-BERGHEI GAMETOCYTES INTO OOKINETES IN-VITRO

被引:25
作者
RANAWAKA, GRR [1 ]
ALEJOBLANCO, AR [1 ]
SINDEN, RE [1 ]
机构
[1] UNIV LONDON IMPERIAL COLL SCI & TECHNOL,DEPT BIOL,MOLEC & CELLULAR PARASITOL RES GRP,LONDON SW7 2BB,ENGLAND
关键词
OOKINETE; TRANSMISSION-BLOCKING ANTIBODY; PHAGOCYTOSIS; LEUKOCYTES;
D O I
10.1017/S0031182000077702
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The transmission-blocking monoclonal antibody 13.1, which recognizes the ookinete surface antigen Pbs21 of Plasmodium berghei, and an IgG2a isotype control antibody 26.37 were purified by caprylic acid and ammonium sulphate precipitation. Fab fragments were prepared by papain digestion. IgG but not Fab from antibody 13.1 reduced ookinete formation by P. berghei in culture by as much as 94% at a concentration of 100 mu g/ml. There was little difference in antibody efficacy in the range 6.25-400 mu g/ml in this assay. The parasite was most sensitive to antibody activity in the first 6-9 h of culture, i.e. the gamete/zygote and early retort stages. Peripheral blood leucocytes (PBL) were essential to achieve maximal inhibition by mAb 13.1 (activity was abrogated totally if PBL were removed). Together the data suggest that one of the mechanisms of action of this antibody is antibody-mediated PBL killing. Phagocytosis of parasites was noted in these experiments in all cultures. We have not attempted in this study to distinguish between Fc-mediated opsonization, as opposed to antibody-dependent cellular cytotoxicity.
引用
收藏
页码:11 / 17
页数:7
相关论文
共 27 条
[1]   AN ELECTRON MICROSCOPICAL STUDY OF THE INTERACTION OF MONOCLONAL-ANTIBODIES WITH GAMETES OF THE MALARIAL PARASITE PLASMODIUM-GALLINACEUM [J].
AIKAWA, M ;
RENER, J ;
CARTER, R ;
MILLER, LH .
JOURNAL OF PROTOZOOLOGY, 1981, 28 (03) :383-388
[2]   RECOMBINANT PFS25 PROTEIN OF PLASMODIUM-FALCIPARUM ELICITS MALARIA TRANSMISSION-BLOCKING IMMUNITY IN EXPERIMENTAL-ANIMALS [J].
BARR, PJ ;
GREEN, KM ;
GIBSON, HL ;
BATHURST, IC ;
QUAKYI, IA ;
KASLOW, DC .
JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 174 (05) :1203-1208
[3]  
BILLINGSLEY PF, 1994, IN PRESS AM J TROPIC
[4]   A POTENTIAL FUSION PEPTIDE AND AN INTEGRIN LIGAND DOMAIN IN A PROTEIN ACTIVE IN SPERM-EGG FUSION [J].
BLOBEL, CP ;
WOLFSBERG, TG ;
TURCK, CW ;
MYLES, DG ;
PRIMAKOFF, P ;
WHITE, JM .
NATURE, 1992, 356 (6366) :248-252
[5]   PROPERTIES OF EPITOPES OF PFS 48/45, A TARGET OF TRANSMISSION BLOCKING MONOCLONAL-ANTIBODIES, ON GAMETES OF DIFFERENT ISOLATES OF PLASMODIUM-FALCIPARUM [J].
CARTER, R ;
GRAVES, PM ;
KEISTER, DB ;
QUAKYI, IA .
PARASITE IMMUNOLOGY, 1990, 12 (06) :587-603
[6]   PGS28 BELONGS TO A FAMILY OF EPIDERMAL GROWTH-FACTOR LIKE ANTIGENS THAT ARE TARGETS OF MALARIA TRANSMISSION-BLOCKING ANTIBODIES [J].
DUFFY, PE ;
PIMENTA, P ;
KASLOW, DC .
JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (02) :505-510
[7]   COMPLEMENT EFFECTS ON THE INFECTIVITY OF PLASMODIUM-GALLINACEUM TO AEDES-AEGYPTI MOSQUITOS .2. CHANGES IN SENSITIVITY TO COMPLEMENT-LIKE FACTORS DURING ZYGOTE DEVELOPMENT [J].
GROTENDORST, CA ;
CARTER, R .
JOURNAL OF PARASITOLOGY, 1987, 73 (05) :980-984
[8]  
GROTENDORST CA, 1986, J IMMUNOL, V136, P4270
[9]   DEVELOPMENT OF PLASMODIUM-BERGHEI OOKINETES IN THE MIDGUT OF ANOPHELES-ATROPARVUS MOSQUITOS AND INVITRO [J].
JANSE, CJ ;
ROUWENHORST, RJ ;
VANDERKLOOSTER, PFJ ;
VANDERKAAY, HJ ;
OVERDULVE, JP .
PARASITOLOGY, 1985, 91 (OCT) :219-225
[10]   MALARIA TRANSMISSION-BLOCKING VACCINES [J].
KASLOW, DC ;
BATHURST, IC ;
BARR, PJ .
TRENDS IN BIOTECHNOLOGY, 1992, 10 (11) :388-391