TRANSACTIVATION AND DNA-BINDING PROPERTIES OF THE TRANSCRIPTION FACTOR, SOX-18

被引：75

作者：

HOSKING, BM ^{[1
]}

MUSCAT, GEO ^{[1
]}

KOOPMAN, PA ^{[1
]}

DOWHAN, DH ^{[1
]}

DUNN, TL ^{[1
]}

机构：

[1] UNIV QUEENSLAND,CTR MOLEC & CELLULAR BIOL,ST LUCIA,QLD 4072,AUSTRALIA

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 14期

关键词：

D O I：

10.1093/nar/23.14.2626

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sox-18 is a member of the Sox multi-gene family (Sry-related HMG-box gene), We have bacterially expressed this 378 amino acid protein and demonstrated sequence-specific binding to the Sox DNA-binding motif AACAAAG. A distinct 95 amino acid activation domain was mapped in Sox-18 using GAL4-Sox-18 fusions (amino acids 160-225). Furthermore, Sox-18 was capable of trans-activating gene expression through the AACAAAG motif. Our results suggest that Sox-18 functions as a classical trans-activator of gene expression.

引用

页码：2626 / 2628

页数：3

共 19 条

[1] GENETIC-EVIDENCE EQUATING SRY AND THE TESTIS-DETERMINING FACTOR
BERTA, P
HAWKINS, JR
SINCLAIR, AH
TAYLOR, A
GRIFFITHS, BL
GOODFELLOW, PN
FELLOUS, M
[J]. NATURE, 1990, 348 (6300) : 448 - 450
[2] DNA-BINDING AND BENDING PROPERTIES OF THE POSTMEIOTICALLY EXPRESSED SRY-RELATED PROTEIN SOX-5
CONNER, F
CARY, PD
READ, CM
PRESTON, NS
DRISCOLL, PC
DENNY, P
CRANEROBINSON, C
ASHWORTH, A
[J]. NUCLEIC ACIDS RESEARCH, 1994, 22 (16) : 3339 - 3346
[3] AN SRY-RELATED GENE EXPRESSED DURING SPERMATOGENESIS IN THE MOUSE ENCODES A SEQUENCE-SPECIFIC DNA-BINDING PROTEIN
DENNY, P
SWIFT, S
CONNOR, F
ASHWORTH, A
[J]. EMBO JOURNAL, 1992, 11 (10) : 3705 - 3712
[4] DOWNES M, 1993, CELL GROWTH DIFFER, V4, P901
[5] SRY IS A TRANSCRIPTIONAL ACTIVATOR
DUBIN, RA
OSTRER, H
[J]. MOLECULAR ENDOCRINOLOGY, 1994, 8 (09) : 1182 - 1192
[6] DUNN T, 1995, IN PRESS GENE, V161
[7] PARTIAL CLONING OF SOX-11 AND SOX-12, 2 NEW HUMAN SOX GENES
GOZE, C
POULAT, F
BERTA, P
[J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (12) : 2943 - 2943
[8] MALE DEVELOPMENT OF CHROMOSOMALLY FEMALE MICE TRANSGENIC FOR SRY
KOOPMAN, P
GUBBAY, J
VIVIAN, N
GOODFELLOW, P
LOVELLBADGE, R
[J]. NATURE, 1991, 351 (6322) : 117 - 121
[9] ANCESTRY AND DIVERSITY OF THE HMG BOX SUPERFAMILY
LAUDET, V
STEHELIN, D
CLEVERS, H
[J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (10) : 2493 - 2501
[10] EVIDENCE FOR PHYSICAL INTERACTION BETWEEN THE ZINC-FINGER TRANSCRIPTION FACTORS YY1 AND SP1
LEE, JS
GALVIN, KM
SHI, Y
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (13) : 6145 - 6149

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