ACTIVATION OF ALPHA-MYOSIN HEAVY-CHAIN GENE-EXPRESSION BY CAMP IN CULTURED FETAL-RAT HEART MYOCYTES

The effect of cAMP on cardiac myosin heavy chain (MHC) gene expression in primary cultures of 18-day-old fetal rat heart myocytes was investigated. When myocytes were treated with either 10 μM forskolin or 1 mM 8-bromo-cAMP for 48 h, the relative amount of the V1 to-V3 myosin isoform ratio increased 3-fold. The abundance of alpha-MHC mRNA was also increased 3-to-4-fold in forskolin treated vs control cells. However, no appreciable change was observed in the level of beta-MHC mRNA. In addition, a 70% increase in the transcription rate of the cardiac MHC gene was observed by nuclear run-on assay following treatment of cells with 10 μM forskolin for 12 h. These results demonstrate the preferential induction of alpha-MHC mRNA by cAMP which is, in part, mediated by transcriptional activation of the gene. © 1991.