RECOVERY OF A RECOMBINANT CUTINASE WITH REVERSED MICELLES IN A CONTINUOUS PERFORATED ROTATING-DISC CONTACTOR

被引：24

作者：

CARNEIRODACUNHA, MG

AIRESBARROS, MR

TAMBOURGI, EB

CABRAL, JMS

机构：

[1] Univ Tecn Lisboa, ENGN BIOQUIM LAB, INST SUPER TECN, P-1000 LISBON, PORTUGAL

[2] UNICAMP, FEQ, DESQ, BR-13081970 CAMPINAS, SP, BRAZIL

来源：

BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 06期

关键词：

D O I：

10.1007/BF00154313

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A continuous perforated rotating disc contactor was used for the extraction of a recombinant cutinase from an aqueous solution to a reversed micellar phase of AOT in isooctane. Cutinase was extracted to the organic phase with protein yield of 78% after 70 minutes of operation.

引用

收藏

页码：413 / 418

页数：6

相关论文

共 13 条

[1] SELECTIVE SEPARATION AND PURIFICATION OF 2 LIPASES FROM CHROMOBACTERIUM-VISCOSUM USING AOT REVERSED MICELLES [J].

AIRESBARROS, MR ;

CABRAL, JMS .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (11) :1302-1307

[2]

CARNEIRODACUNHA MG, 1994, IN PRESS BIOPROCESS

[3] ENZYME RECOVERY BY LIQUID-LIQUID-EXTRACTION USING REVERSED MICELLES [J].

DEKKER, M ;

VANTRIET, K ;

WEIJERS, SR ;

BALTUSSEN, JWA ;

LAANE, C ;

BIJSTERBOSCH, BH .

CHEMICAL ENGINEERING JOURNAL AND THE BIOCHEMICAL ENGINEERING JOURNAL, 1986, 33 (02) :B27-B33

[4] LIQUID-LIQUID-EXTRACTION OF LOW-MOLECULAR-WEIGHT PROTEINS BY SELECTIVE SOLUBILIZATION IN REVERSED MICELLES [J].

GOKLEN, KE ;

HATTON, TA .

SEPARATION SCIENCE AND TECHNOLOGY, 1987, 22 (2-3) :831-841

[5] RAPID COLORIMETRIC DETERMINATION OF FREE FATTY-ACIDS [J].

LOWRY, RR ;

TINSLEY, IJ .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1976, 53 (07) :470-472

[6] FUSARIUM-SOLANI CUTINASE IS A LIPOLYTIC ENZYME WITH A CATALYTIC SERINE ACCESSIBLE TO SOLVENT [J].

MARTINEZ, C ;

DEGEUS, P ;

LAUWEREYS, M ;

MATTHYSSENS, G ;

CAMBILLAU, C .

NATURE, 1992, 356 (6370) :615-618

[7]

MELO EP, 1992, OCT CEC INT WORKSH L

[8] LIQUID-LIQUID-EXTRACTION OF A RECOMBINANT PROTEIN WITH A REVERSE MICELLE PHASE [J].

PIRES, MJ ;

CABRAL, JMS .

BIOTECHNOLOGY PROGRESS, 1993, 9 (06) :647-650

[9] RECOVERY OF AN EXTRACELLULAR ALKALINE PROTEASE FROM WHOLE FERMENTATION BROTH USING REVERSED MICELLES [J].

RAHAMAN, RS ;

CHEE, JY ;

CABRAL, JMS ;

HATTON, TA .

BIOTECHNOLOGY PROGRESS, 1988, 4 (04) :218-224

[10]

REMAN GH, 1955, GENIE CHIM, V74, P106