SYNTHESIS AND PROPERTIES OF OLIGONUCLEOTIDES CONTAINING THE MUTAGENIC BASE O-4-BENZYLTHYMIDINE

被引：8

作者：

FABREGA, C

ERITJA, R

SINHA, ND

DOSANJH, MK

SINGER, B

机构：

[1] CSIC, CID, DEPT MOLEC GENET, E-08034 BARCELONA, Spain

[2] EUROPEAN MOLEC BIOL LAB, D-69117 HEIDELBERG, Germany

[3] MILLIPORE CORP, LIFE SCI RES GRP, BEDFORD, MA 01730 USA

[4] UNIV CALIF BERKELEY, LAWRENCE BERKELEY LAB, DONNER LAB, DIV LIFE SCI, BERKELEY, CA 94720 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1995年 / 3卷 / 01期

关键词：

D O I：

10.1016/0968-0896(94)00148-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation of synthetic oligodeoxynucleotides containing O-4-benzylthymidine (T-bn) is described. The use of standard and t-butylphenoxyacetyl amino protecting groups is compared. The thermal stabilities of duplexes containing T-bn paired with adenine and guanine have been measured.

引用

收藏

页码：101 / 108

页数：8

相关论文

共 23 条

[1] SOLID-PHASE SYNTHESIS AND SIDE REACTIONS OF OLIGONUCLEOTIDES CONTAINING O-ALKYLTHYMINE RESIDUES [J].

BOROWYBOROWSKI, H ;

CHAMBERS, RW .

BIOCHEMISTRY, 1989, 28 (04) :1471-1477

[2] USE OF A DODECADEOXYNUCLEOTIDE TO STUDY REPAIR OF THE O-4-METHYLTHYMINE LESION [J].

DOLAN, ME ;

OPLINGER, M ;

PEGG, AE .

MUTATION RESEARCH, 1988, 193 (02) :131-137

[3] BOTH O4-METHYLTHYMINE AND O4-ETHYLTHYMINE PREFERENTIALLY FORM ALKYL T-G PAIRS THAT DO NOT BLOCK IN-VITRO REPLICATION IN A DEFINED SEQUENCE [J].

DOSANJH, MK ;

MENICHINI, P ;

ERITJA, R ;

SINGER, B .

CARCINOGENESIS, 1993, 14 (09) :1915-1919

[4] EVIDENCE FROM INVITRO REPLICATION THAT O6-METHYLGUANINE CAN ADOPT MULTIPLE CONFORMATIONS [J].

DOSANJH, MK ;

LOECHLER, EL ;

SINGER, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (09) :3983-3987

[5]

Eckstein F., 1991, OLIGONUCLEOTIDES ANA

[6] SYNTHESIS AND CHARACTERIZATION OF OLIGODEOXYNUCLEOTIDES CONTAINING THE MUTAGENIC BASE ANALOG 4-O-ETHYLTHYMINE [J].

FERNANDEZFORNER, D ;

PALOM, Y ;

IKUTA, S ;

PEDROSO, E ;

ERITJA, R .

NUCLEIC ACIDS RESEARCH, 1990, 18 (19) :5729-5734

[7]

KALNIK MW, 1988, BIOCHEMISTRY-US, V27, P108, DOI 10.1021/bi00401a018

[8] BASE PAIR MISMATCHES AND CARCINOGEN-MODIFIED BASES IN DNA - AN NMR-STUDY OF A.C AND A.O4MET PAIRING IN DODECANUCLEOTIDE DUPLEXES [J].

KALNIK, MW ;

KOUCHAKDJIAN, M ;

LI, BFL ;

SWANN, PF ;

PATEL, DJ .

BIOCHEMISTRY, 1988, 27 (01) :100-108

[9] SYNTHESIS AND CHARACTERIZATION OF OLIGODEOXYNUCLEOTIDES CONTAINING 4-O-METHYLTHYMINE [J].

LI, BFL ;

REESE, CB ;

SWANN, PF .

BIOCHEMISTRY, 1987, 26 (04) :1086-1093

[10]

Pegg A E, 1977, Adv Cancer Res, V25, P195, DOI 10.1016/S0065-230X(08)60635-1