GLUCOCORTICOID INDUCTION OF PULMONARY MATURATION IN THE RABBIT FETUS - EFFECT OF MATERNAL INJECTION OF BETAMETHASONE ON THE ACTIVITY OF ENZYMES IN FETAL LUNG

被引:47
作者
POSSMAYER, F
CASOLA, P
CHAN, F
HILL, S
METCALFE, IL
STEWARTDEHAAN, PJ
WONG, T
HERAS, JL
GAMMAL, EB
HARDING, PGR
机构
[1] UNIV WESTERN ONTARIO,DEPT BIOCHEM,LONDON N6A 3K7,ONTARIO,CANADA
[2] UNIV WESTERN ONTARIO,DEPT PATHOL,LONDON N6A 3K7,ONTARIO,CANADA
[3] UNIV WESTERN ONTARIO,DEPT ANAT,LONDON N6A 3K7,ONTARIO,CANADA
基金
英国医学研究理事会;
关键词
(Rabbit fetus); Glucocorticoid induction; Maturation; Phosphatidylcholine; Phosphatidylglycerol; Pulmonary surfactant; Respiratory distresss syndrome;
D O I
10.1016/0005-2760(79)90002-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. 1. Maternal administration of betamethasone (0.2 mg/kg) on day 25 or 26 of gestation produced a significant decrease in the lung/body weight ratio of the rabbit fetuses within 24 h. 2. 2. The incorporation of [14C]choline but not [14C]ethanolamine into the lipids of fetal lung slices was significantly increased, indicating that there was a specific effect on phosphatidylcholine synthesis. 3. 3. The activities of a number of marker enzymes for subcellular organelles were elevated, especially in the lungs of fetuses delivered on day 26. The increases in monoamine oxidase (mitochondrial outer membrane), β-glycerophosphatase and aqueously dispersed phosphatidic acid-dependent phosphatidic acid phosphohydrolase (lysosomal) activities were significant. 4. 4. Although the activity of cholinephosphotransferase was not affected by glucocorticoid treatment, the activities of glycerol-3-phosphate phosphatidyl-transferase and the activities of two enzymes in the auxiliary pathways for the production of disaturated phosphatidylcholine (lysophosphatidylcholine: lysophosphatidylcholine transacylase and lysophosphatidylcholine racyl-CoA acyl-transferase) were significantly increased. 5. 5. Membrane-bound phosphatidic acid-dependent phosphatidic acid phosphohydrolase activity was elevated to a lesser extent than the aqueously dispersed phosphatidate-dependent activity and this increase was not significant. 6. 6. The incorporation of [35S]methionine into protein by slices of fetal lung was significantly reduced after maternal treatment with betamethasone. 7. 7. These results are consistent with the general view that glucocorticoids can induce pulmonary maturation and surfactant production in the rabbit fetus but indicate that some of the former hypotheses regarding the mechanism by which lipid synthesis is accelerated must be reevaluated. © 1979.
引用
收藏
页码:197 / 211
页数:15
相关论文
共 36 条
[11]  
HALLMAN M, 1977, J PERINAT MED, V5, P3
[12]   RATE-LIMITING STEPS IN CYTIDINE PATHWAY FOR SYNTHESIS OF PHOSPHATIDYLCHOLINE AND PHOSPHATIDYLETHANOLAMINE [J].
INFANTE, JP .
BIOCHEMICAL JOURNAL, 1977, 167 (03) :847-849
[13]   FETAL LUNG MATURATION .4. RELEASE OF PHOSPHATIDIC-ACID PHOSPHOHYDROLASE AND PHOSPHOLIPIDS INTO HUMAN AMNIOTIC-FLUID [J].
JIMENEZ, JM ;
JOHNSTON, JM .
PEDIATRIC RESEARCH, 1976, 10 (08) :767-769
[14]   FETAL LUNG MATURATION .2. PHOSPHATIDIC-ACID PHOSPHOHYDROLASE IN HUMAN AMNIOTIC-FLUID [J].
JIMENEZ, JM ;
SCHULTZ, FM ;
MACDONALD, PC ;
JOHNSTON, JM .
GYNECOLOGIC INVESTIGATION, 1974, 5 (5-6) :245-251
[15]   PREPARATION AND CHARACTERIZATION OF TOTAL, ROUGH AND SMOOTH MICROSOMES FROM LUNG OF CONTROL AND METHYLCHOLANTHRENE-TREATED RATS [J].
JOHANNESEN, K ;
DEPIERRE, JW ;
BERGSTRAND, A ;
DALLNER, G ;
ERNSTER, L .
BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 496 (01) :115-135
[16]  
KIKKAWA Y, 1971, AM J PATHOL, V64, P423
[17]  
LIGGINS GC, 1972, PEDIATRICS, V50, P515
[18]   MEASUREMENT OF INCORPORATION OF RADIOACTIVE AMINO ACIDS INTO PROTEIN BY A FILTER-PAPER DISK METHOD [J].
MANS, RJ ;
NOVELLI, GD .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1961, 94 (01) :48-&
[19]  
MAVIS RD, 1978, J LIPID RES, V19, P467