ELECTRON-MICROSCOPIC STUDY ON THE QUATERNARY STRUCTURE OF THE ISOLATED PARTICULATE ALCOHOL-ACETALDEHYDE DEHYDROGENASE COMPLEX AND ON ITS IDENTITY WITH THE POLYGONAL BODIES OF CLOSTRIDIUM-KLUYVERI

被引:23
作者
LURZ, R [1 ]
MAYER, F [1 ]
GOTTSCHALK, G [1 ]
机构
[1] UNIV GOTTINGEN, INST MIKROBIOL, D-3400 GOTTINGEN, GERMANY
关键词
Acetaldehyde dehydrogenase; Alcohol dehydrogenase; Clostridium kluyveri; Electron microscopy; Enzyme complex; Polygonal bodies;
D O I
10.1007/BF00423073
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The alcohol-acetaldehyde dehydrogenase complex of Clostridium kluyveri has been separated from contaminating β-hydroxybutyryl-CoA dehydrogenase by repeated precipitation with manganese and ammonium sulfate. Mn++ was required for maximum alcohol dehydrogenase activity. The molecular weight of the enzyme complex was 194,000 as determined by sucrose density gradient centrifugation. The enzyme complex has been shown to contain two types of subunits with molecular weights of 55,000±2,600 and 42,000±1,200, respectively which are arranged in H"-shaped particles. In solutions with an ionic strength above 25 mM the enzyme complex precipitated in the form of lumps as has been shown with specific ferritin-conjugated antibodies. These lumps are assumed to be aggregated polygonal bodies present in C. kluyveri. © 1979 Springer-Verlag."
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页码:255 / 262
页数:8
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