CHARACTERIZATION OF GLUTATHIONE TRANSFERASE FROM GAMMARUS-ITALICUS

被引：9

作者：

ACETO, A

DIILIO, C

BUCCIARELLI, T

PANTANI, C

DELLAGATA, M

PANNUNZIO, G

FEDERICI, G

机构：

[1] UNIV ROMA TOR VERGATA,DIPARTIMENTO BIOL,I-00173 ROME,ITALY

[2] UNIV LAQUILA,DIPARTIMENTO SCI AMBIENTALI,I-67100 LAQUILA,ITALY

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1991年 / 99卷 / 03期

关键词：

D O I：

10.1016/0305-0491(91)90332-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. By using affinity chromatography and chromatofocusing analysis at least two major glutathione transferases, named GST II and GST III can be isolated from Gammarus italicus. 2. GST II has an isoelectric point at pH 5.0 and is composed of two subunits with an apparent molecular mass of 28 KDa. 3. GST III which has an isoelectric point at pH 4.6 was found to be an heterodimer of 27 KDa and 28 KDa. 4. The 28 KDa subunit cross-reacted in immunoblotting analysis with antisera raised against pi class GST, whereas none of the antisera raised against alpha, mu and pi class GSTs cross-reacted with the 27 KDa subunit.

引用

页码：523 / 527

页数：5

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