NUCLEAR PHOSPHOINOSITIDE SIGNALING ENZYME IN HUMAN B-LYMPHOID CELLS

被引：9

作者：

CATALDI, A ^{[1
]}

DIPIETRO, R ^{[1
]}

ROBUFFO, I ^{[1
]}

DIBALDASSARRE, A ^{[1
]}

MISCIA, S ^{[1
]}

机构：

[1] UNIV G ANNUNZIO, IST MORFOL UMANA NORMALE, I-66100 CHIETI, ITALY

来源：

CELL STRUCTURE AND FUNCTION | 1994年 / 19卷 / 06期

关键词：

INTERFERON; PHOSPHOINOSITIDASE C; NUCLEUS; BURKITT LYMPHOMA CELLS;

D O I：

10.1247/csf.19.375

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The modulation of phosphoinositidase C (PIG) beta activity upon interferon treatment in Burkitt lymphoma cells (Daudi) and its localization and expression have been analyzed by Western blotting, immunocytochemical and immunoelectronmicroscopy analysis. Results have disclosed an early increase of phos-phatidyl-inositol-bisphosphate (PIP2) hydrolysis at nuclear level upon interferon (IFN) treatment paralleled by the evidence of an increase of PIC beta 1 expression. PIC beta 1 expression has been detected in the nuclear compartment also in a clone of Daudi cells selected for the resistance to the antiproliferative action of interferon alpha but no modulation of the enzyme has been detected upon interferon treatment. Since no changes in terms of PIP2 hydrolysis have been found at nuclear level in this selected line, we suggest that the antiproliferative action of interferon on Burkitt lymphoma cells is mediated by a possible recruitment of nuclear PIC beta 1 expression.

引用

页码：375 / 384

页数：10

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