IMMUNOHISTOLOGICAL LOCALIZATION OF TRYPSIN IN MUCUS-SECRETING CELL-LAYERS OF ATLANTIC SALMON, SALMO-SALAR L

被引：49

作者：

BRAUN, R

ARNESEN, JA

RINNE, A

HJELMELAND, K

机构：

[1] Institute of Medical Biology, University of Tromsø, Tromsø

[2] Foundation of Applied Research, University of TromsØ, Tromsø

来源：

JOURNAL OF FISH DISEASES | 1990年 / 13卷 / 03期

关键词：

D O I：

10.1111/j.1365-2761.1990.tb00778.x

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Abstract. Immunohistochcmical examination showed that trypsin was present in mucus‐secreting cell layers of Atlantic salmon, such as surface epithelial cells of gills and intestine, and epidermal cells of dorsal skin. Trypsin in tissue slices was identified by an immunohisto‐chemical technique which used affinity purified immunoglobulins from rabbit antisera against purified salmon pancreatic trypsin as primary antibodies. Most of the positively stained cells appeared to be granulated and secretory. The authors hypothesize that trypsin in mucus‐secreting cell layers is a part of the non‐specific immune defence of the fish. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：233 / 238

页数：6

共 16 条

[1]

BRADSHAW CM, 1971, P SOC EXP BIOL MED, V136, P1122

[2]

DICONZA JJ, 1971, AUST J EXP BIOL MED, V49, P517

[3]

Fletcher T.C., 1978, Biochemical and Biophysical Perspectives in Marine Biology, V4, P189

[4]

FLETCHER TC, 1973, EXPERIENTIA, V29, P1282

[5]

FLETCHER TC, 1968, BIOCHEM J, V18, P12

[6] HUMORAL-FACTORS IMPORTANT IN RESISTANCE OF SALMONID FISH TO BACTERIAL DISEASE .2. ANTI-VIBRIO ANGUILLARUM ACTIVITY IN MUCUS AND OBSERVATIONS ON COMPLEMENT [J].

HARRELL, LW ;

ETLINGER, HM ;

HODGINS, HO .

AQUACULTURE, 1976, 7 (04) :363-370

[7]

Harris J.E., 1972, International J Parasit, V2, P459, DOI 10.1016/0020-7519(72)90091-4

[8] PROTEINASE-INHIBITORS IN THE MUSCLE AND SERUM OF COD (GADUS-MORHUA) - ISOLATION AND CHARACTERIZATION [J].

HJELMELAND, K .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1983, 76 (02) :365-372

[9] CHARACTERISTICS OF 2 TRYPSIN TYPE ISOZYMES ISOLATED FROM THE ARCTIC FISH CAPELIN (MALLOTUS-VILLOSUS) [J].

HJELMELAND, K ;

RAA, J .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1982, 71 (04) :557-562

[10] SUBSTANCES INVOLVED IN THE NATURAL-RESISTANCE OF FISH TO INFECTION - A REVIEW [J].

INGRAM, GA .

JOURNAL OF FISH BIOLOGY, 1980, 16 (01) :23-60

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