ISOLATION AND OVEREXPRESSION IN ESCHERICHIA-COLI OF THE FLAVODOXIN GENE FROM ANABAENA-PCC-7119

被引：79

作者：

FILLAT, MF ^{[1
]}

BORRIAS, WE ^{[1
]}

WEISBEEK, PJ ^{[1
]}

机构：

[1] UNIV UTRECHT, DEPT MOLEC CELL BIOL, 3584 CH UTRECHT, NETHERLANDS

来源：

BIOCHEMICAL JOURNAL | 1991年 / 280卷

关键词：

D O I：

10.1042/bj2800187

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene coding for flavodoxin from Anabaena PCC 7119 was cloned by using the polymerase chain reaction (PCR). The gene is transcribed into a 1250-base transcript. The expression of the flavodoxin gene was analysed and found to be regulated at the transcriptional level by the availability of iron. The PCR-amplified gene was cloned into the expression vector pTrc 99b and expressed in Escherichia coli. High concentrations of flavodoxin were found (20% of total protein). The recombinant protein was purified from the cytosolic fraction of the cells and it exhibited properties identical with those of the wild-type Anabaena flavodoxin.

引用

页码：187 / 191

页数：5

共 35 条

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