GENETIC APPROACH TO FACILITATE PURIFICATION OF RECOMBINANT PROTEINS WITH A NOVEL METAL CHELATE ADSORBENT

被引：1021

作者：

HOCHULI, E

BANNWARTH, W

DOBELI, H

GENTZ, R

STUBER, D

机构：

来源：

BIO-TECHNOLOGY | 1988年 / 6卷 / 11期

关键词：

D O I：

10.1038/nbt1188-1321

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

引用

页码：1321 / 1325

页数：5

共 18 条

[1] ESCHERICHIA-COLI DIHYDROFOLATE-REDUCTASE - ISOLATION AND CHARACTERIZATION OF 2 ISOZYMES [J].

BACCANARI, DP ;

AVERETT, D ;

BRIGGS, C ;

BURCHALL, J .

BIOCHEMISTRY, 1977, 16 (16) :3566-3572

[2] A SYSTEM FOR THE SIMULTANEOUS CHEMICAL SYNTHESIS OF DIFFERENT DNA FRAGMENTS ON SOLID SUPPORT [J].

BANNWARTH, W ;

IAIZA, P .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1986, 5 (05) :413-419

[3] GENE ORGANIZATION AND PRIMARY STRUCTURE OF A RIBOSOMAL-RNA OPERON FROM ESCHERICHIA-COLI [J].

BROSIUS, J ;

DULL, TJ ;

SLEETER, DD ;

NOLLER, HF .

JOURNAL OF MOLECULAR BIOLOGY, 1981, 148 (02) :107-127

[4]

BUJARD H, 1987, METHOD ENZYMOL, V155, P416

[5] SUBREGIONS OF A CONSERVED PART OF THE HIV-GP41 TRANSMEMBRANE PROTEIN ARE DIFFERENTIALLY RECOGNIZED BY ANTIBODIES OF INFECTED INDIVIDUALS [J].

CERTA, U ;

BANNWARTH, W ;

STUBER, D ;

GENTZ, R ;

LANZER, M ;

LEGRICE, S ;

GUILLOT, F ;

WENDLER, I ;

HUNSMANN, G ;

BUJARD, H ;

MOUS, J .

EMBO JOURNAL, 1986, 5 (11) :3051-3056

[6] PHENOTYPIC EXPRESSION IN ESCHERICHIA-COLI OF A DNA-SEQUENCE CODING FOR MOUSE DIHYDROFOLATE-REDUCTASE [J].

CHANG, ACY ;

NUNBERG, JH ;

KAUFMAN, RJ ;

ERLICH, HA ;

SCHIMKE, RT ;

COHEN, SN .

NATURE, 1978, 275 (5681) :617-624

[7] MAJOR SURFACE-ANTIGEN P190 OF PLASMODIUM-FALCIPARUM - DETECTION OF COMMON EPITOPES PRESENT IN A VARIETY OF PLASMODIA ISOLATES [J].

GENTZ, R ;

CERTA, U ;

TAKACS, B ;

MATILE, H ;

DOBELI, H ;

PINK, R ;

MACKAY, M ;

BONE, N ;

SCAIFE, JG .

EMBO JOURNAL, 1988, 7 (01) :225-230

[8] RAPID PURIFICATION OF A CLONED GENE-PRODUCT BY GENETIC FUSION AND SITE-SPECIFIC PROTEOLYSIS [J].

GERMINO, J ;

BASTIA, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (15) :4692-4696

[9] NEW METAL CHELATE ADSORBENT SELECTIVE FOR PROTEINS AND PEPTIDES CONTAINING NEIGHBORING HISTIDINE-RESIDUES [J].

HOCHULI, E ;

DOBELI, H ;

SCHACHER, A .

JOURNAL OF CHROMATOGRAPHY, 1987, 411 :177-184

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

← 1 2 →