A COMPLEX PATTERN OF H2A PHOSPHORYLATION IN THE MOUSE TESTIS

被引：13

作者：

GREEN, GR

PATEL, JC

HECHT, NB

POCCIA, DL

机构：

[1] MT HOLYOKE COLL,DEPT BIOL,S HADLEY,MA 01075

[2] TUFTS UNIV,DEPT BIOL,MEDFORD,MA 02155

来源：

EXPERIMENTAL CELL RESEARCH | 1991年 / 195卷 / 01期

关键词：

D O I：

10.1016/0014-4827(91)90493-E

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Phosphorylation of H2A histones in mouse testis was examined using testis tubule cultures labeled with 32PO4. Histones were analyzed by two systems of two-dimensional polyacrylamide gel electrophoresis, followed by autoradiography of the gels. Of the 32PO4 detected in histones, 95% was incorporated by certain modified forms of the H2A variants H2A.1 and H2A.X. Phosphorylation sites were mapped to N- and C-terminal regions of the modified variants by SDS gel electrophoresis and autoradiography of peptides generated by cleavage of in vitro-labeled proteins with N-bromosuccinimide. Incorporation rates differed for N- and C-terminal regions from different modified forms, demonstrating a complex pattern of H2A phosphorylation in the mouse testis. © 1991.

引用

页码：8 / 12

页数：5

共 31 条

[11]

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