REGULATION OF THE PRODUCTION OF THE AGGLUTINATION SUBSTANCES RESPONSIBLE FOR SEXUAL AGGLUTINATION IN SACCHAROMYCES-CEREVISIAE - CHANGES ASSOCIATED WITH CONJUGATION AND TEMPERATURE SHIFT

Isolated zygotes showed self-agglutination caused by the sex-specific glycoproteins, the agglutination substances responsible for sexual agglutination. The agglutination substances of both a and α mating types were detected in the extracts obtained by the autoclave method from zygotes. Although the first diploid daughter cells from zygotes showed self-agglutinability, the self-agglutinability decreased gradually in the successive diploid daughter cells. The self-agglutination in diploid cells was also brought about by the complementary binding of the sex-specific agglutination substances of opposite mating types. The constitutive sexual agglutinability in a and α cells was lost with concomitant loss of the agglutination substances in both cell wall and cytoplasmic fractions when cultured at a temperature higher than 35°C. The repression of the production of the agglutination substances was reversed by the opposite mating type pheromones even at the repressive temperature, 36°C, associated with the appearance of sexual agglutinability. The sex pheromones, a substance-I and α substance-I, and the binding substance for α substance-I were produced even at 36°C, repressive for the production of the agglutination substances. © 1979 Springer-Verlag.