POLYPEPTIDES FROM THE MYXOMYCETE PHYSARUM-POLYCEPHALUM INTERACTING INVITRO WITH MICROTUBULES

被引：5

作者：

ALBERTINI, C ^{[1
]}

AKHAVANNIAKI, H ^{[1
]}

WRIGHT, M ^{[1
]}

机构：

[1] CNRS, PHARMACOL & TOXICOL FONDAMENTALES LAB, 205 ROUTE NARBONNE, F-31077 TOULOUSE, FRANCE

来源：

CELL MOTILITY AND THE CYTOSKELETON | 1990年 / 17卷 / 04期

关键词：

MICROTUBULE-ASSOCIATED PROTEINS; CELL CYCLE; PHOSPHORYLATION;

D O I：

10.1002/cm.970170402

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Microtubule-interacting proteins have been studied in the lower eukaryote Physarum polycephalum. We show for the first time 1) the presence in Physarum amoebal crude extracts of at least six polypeptides that bind specifically to amoebal microtubules, 2) the binding between these proteins and mammalian microtubules, 3) the heat stability of two of these polypeptides (125 and 235 kDa), 4) the functional properties of a fraction containing a heat-soluble 125 kDa polypeptide, and 5) the phosphorylation of the 125 kDa polypeptide during two distinct periods of the cell cycle in Physarum synchronous plasmodia, first at late S/early G2 phase and second at late G2/prophase.

引用

页码：267 / 275

页数：9

共 40 条

[31]

THEURKAUF WE, 1983, J BIOL CHEM, V258, P7883

[32] IDENTIFICATION OF A NOVEL FORCE-GENERATING PROTEIN, KINESIN, INVOLVED IN MICROTUBULE-BASED MOTILITY [J].

VALE, RD ;

REESE, TS ;

SHEETZ, MP .

CELL, 1985, 42 (01) :39-50

[33]

Vallee R B, 1984, Cell Muscle Motil, V5, P289

[34] ON THE USE OF HEAT-STABILITY AS A CRITERION FOR THE IDENTIFICATION OF MICROTUBULE ASSOCIATED PROTEINS (MAPS) [J].

VALLEE, RB .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 133 (01) :128-133

[35] A TAXOL-DEPENDENT PROCEDURE FOR THE ISOLATION OF MICROTUBULES AND MICROTUBULE-ASSOCIATED PROTEINS (MAPS) [J].

VALLEE, RB .

JOURNAL OF CELL BIOLOGY, 1982, 92 (02) :435-442

[36] GLYCOLYTIC ENZYME INTERACTIONS WITH TUBULIN AND MICROTUBULES [J].

WALSH, JL ;

KEITH, TJ ;

KNULL, HR .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 999 (01) :64-70

[37] PROTEIN FACTOR ESSENTIAL FOR MICROTUBULE ASSEMBLY [J].

WEINGARTEN, MD ;

LOCKWOOD, AH ;

HWO, SY ;

KIRSCHNER, MW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (05) :1858-1862

[38]

WHEALS AE, 1970, GENETICS, V66, P623

[39] HEAT-SENSITIVE FACTOR NECESSARY FOR MITOSIS ONSET IN PHYSARUM-POLYCEPHALUM (TEMPERATURE SHIFT-HEAT SHOCK-CYCLOHEXIMIDE-TS MUTANT [J].

WRIGHT, M ;

TOLLON, Y .

MOLECULAR & GENERAL GENETICS, 1978, 163 (01) :91-99

[40] MICROTUBULE CYTOSKELETON AND MORPHOGENESIS IN THE AMEBAS OF THE MYXOMYCETE PHYSARUM-POLYCEPHALUM [J].

WRIGHT, M ;

ALBERTINI, C ;

PLANQUES, V ;

SALLES, I ;

DUCOMMUN, B ;

GELY, C ;

AKHAVANNIAKI, H ;

MIR, L ;

MOISAND, A ;

OUSTRIN, ML .

BIOLOGY OF THE CELL, 1988, 63 (02) :239-248

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