BRUCELLA-ABORTUS CYTOPLASMIC PROTEINS USED AS ANTIGENS IN AN ELISA POTENTIALLY USEFUL FOR THE DIAGNOSIS OF CANINE BRUCELLOSIS

被引:24
作者
BALDI, PC
WANKE, MM
LOZA, ME
FOSSATI, CA
机构
[1] UNIV BUENOS AIRES,CONICET,IDEHU,INST ESTUD INMUNIDAD HUMORAL,RA-1113 BUENOS AIRES,ARGENTINA
[2] FAC CIENCIAS VETE BUENOS AIRES,SERV OBSTET,RA-1417 BUENOS AIRES,DF,ARGENTINA
关键词
BRUCELLA ABORTUS; CANINE; CYTOPLASMIC PROTEIN; LPS; O ANTIGEN;
D O I
10.1016/0378-1135(94)90142-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A preparation of Brucella abortus cytoplasmic proteins was depleted of lipopolisaccharyde (LPS) by immunoadsorption with a monoclonal antibody (BC68) specific for the O antigen of B. abortus smooth LPS. This extract was used as antigen in an indirect ELISA for measuring antiprotein humoral immune response in dogs suffering from brucellosis and in healthy controls. All of the affected dogs studied showed IgG antiprotein response, while 2% (2 of 103) of the healthy dogs used as controls gave a positive result. All sera found to be positive by ELISA were also positive by the rapid slide agglutination test. These preliminary results show that the ELISA using B. abortus cytoplasmic proteins could be useful for the specific diagnosis of canine brucellosis.
引用
收藏
页码:127 / 134
页数:8
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