OBF-1, A NOVEL B-CELL-SPECIFIC COACTIVATOR THAT STIMULATES IMMUNOGLOBULIN PROMOTER ACTIVITY THROUGH ASSOCIATION WITH OCTAMER-BINDING PROTEINS

被引:357
作者
STRUBIN, M [1 ]
NEWELL, JW [1 ]
MATTHIAS, P [1 ]
机构
[1] FRIEDRICH MIESCHER INST,CH-4002 BASEL,SWITZERLAND
关键词
D O I
10.1016/0092-8674(95)90500-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recent biochemical and genetic studies indicate that in addition to the octamer-binding proteins Oct-1 and Oct-2, other B cell components are required for lymphoid-restricted, octamer site-mediated immunoglobulin gene promoter activity. Using a genetic screen in yeast, we have isolated a cell-derived cDNAs encoding Oct-binding factor 1 (OBF-1), a novel protein that specifically associates with Oct-1 and Oct-2. Biochemical studies demonstrate that OBF-1 has no intrinsic DNA-binding activity and recognizes the POU domains of Oct-1 and Oct-2, but not those of Oct-4 and Oct-6. The OBF-1 mRNA is expressed in a highly cell-specific manner, being most abundant in B cells and essentially absent In most of the other cells or tissues tested. Furthermore, expression of OBF-1 in HeLa cells selectively stimulates the activity of a natural immunoglobulin promoter in an octamer site-dependent manner. Thus, OBF-1 has all the properties expected for a B cell-specific transcriptional coactivator protein.
引用
收藏
页码:497 / 506
页数:10
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