PURIFICATION AND PROPERTIES OF PIG BRAIN CATECHOL-ORTHO-METHYLTRANSFERASE

被引：6

作者：

GULLIVER, PA ^{[1
]}

TIPTON, KF ^{[1
]}

机构：

[1] UNIV CAMBRIDGE, DEPT BIOCHEM, CAMBRIDGE CB2 1QW, ENGLAND

来源：

JOURNAL OF NEUROCHEMISTRY | 1979年 / 32卷 / 05期

关键词：

D O I：

10.1111/j.1471-4159.1979.tb11094.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— The previously reported affinity chromatography technique (Gulliver & Tipton, 1978b) has been adapted for the large‐scale purification of pig brain catechol‐o‐methyltransfcrase. The enzyme prepared by this method is apparently homogeneous by polyacrylamide gel electrophoresis criteria and is stable for prolonged periods of storage at—5°C in 20% (v/v) glycerol. Kinetic measurements, molecular weight criteria and antiserum reaction suggest that the brain and liver forms of catechol‐o‐methyltransferase are very closely related, if not identical. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：1525 / 1529

页数：5

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