IDENTIFICATION OF MITOSIS-SPECIFIC P65 DIMER AS A COMPONENT OF HUMAN-PHASE-PROMOTING FACTOR

被引：20

作者：

MEIKRANTZ, W

SUPRYNOWICZ, FA

HALLECK, MS

SCHLEGEL, RA

机构：

[1] PENN STATE UNIV,DEPT MOLEC & CELL BIOL,UNIVERSITY PK,PA 16802

[2] NCI,BIOCHEM LAB,BETHESDA,MD 20892

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 24期

关键词：

cyclin B; histone H1 kinase; p34(cdc2); sulfhydryl cycle;

D O I：

10.1073/pnas.87.24.9600

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Antisera raised against two mitosis-specific protein kinases from human cells recognized a single 65-kDa polypeptide (p65) that is present in similar amounts in interphase and mitotic cell extracts. Immunoblot analysis of reduced and unreduced extracts revealed that p65 exists as a 65-kDa monomer during interphase but forms a 130-kDa disulfide-linked homodimer during mitosis. Several different antibodies recognizing the p34(cdc2) protein kinase and cyclin B components of M phase-promoting factor (MPF) coprecipitated p65 from mitotic but not from interphase extracts. In addition, an anti-p65 immunoaffinity column substantially depleted mitotic extracts of histone H1 kinase activity assayed under conditions diagnostic for MPF. These results suggest that active human MPF may be a complex of p34(cdc2), cyclin B, and dimeric p65. A sulfhydryl cycle, proposed in the earlier literature on the biochemistry of mitosis, might underlie the dimerization of p65 and formation of active MPF.

引用

页码：9600 / 9604

页数：5

共 34 条

[31] THE CELL-CYCLE CONTROL GENE CDC2+ OF FISSION YEAST ENCODES A PROTEIN-KINASE POTENTIALLY REGULATED BY PHOSPHORYLATION [J].