MEASLES-VIRUS PHOSPHOPROTEIN (P) REQUIRES THE NH2-TERMINAL AND COOH-TERMINAL DOMAINS FOR INTERACTIONS WITH THE NUCLEOPROTEIN (N) BUT ONLY THE COOH TERMINUS FOR INTERACTIONS WITH ITSELF

被引:77
作者
HARTY, RN [1 ]
PALESE, P [1 ]
机构
[1] MT SINAI SCH MED,DEPT MICROBIOL,NEW YORK,NY 10029
关键词
D O I
10.1099/0022-1317-76-11-2863
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A mammalian two-hybrid system was used to characterize protein-protein interactions between the measles virus nucleoprotein (N) and phosphoprotein (P). Progressive deletions at both the amino- and carboxy-termini of P facilitated the mapping of two distinct domains on P that are important for interaction with N: (i) a domain mapping predominantly within the C-terminal 100 amino acids and (ii) a domain composed of the extreme amino-terminal residues. Using the same two-hybrid assay, we discovered that the P protein interacts strongly with itself. In contrast to the N-P interaction, only a single C-proximal domain of P was essential for P-P interaction.
引用
收藏
页码:2863 / 2867
页数:5
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共 20 条
[11]   SENDAI VIRUS PROTEIN-PROTEIN INTERACTIONS STUDIED BY A PROTEIN-BLOTTING PROTEIN-OVERLAY TECHNIQUE - MAPPING OF DOMAINS ON NP-PROTEIN REQUIRED FOR BINDING TO P-PROTEIN [J].
HOMANN, HE ;
WILLENBRINK, W ;
BUCHHOLZ, CJ ;
NEUBERT, WJ .
JOURNAL OF VIROLOGY, 1991, 65 (03) :1304-1309
[12]   SYNTHESIS OF LEADER RNA AND EDITING OF THE P-MESSENGER-RNA DURING TRANSCRIPTION BY PURIFIED MEASLES-VIRUS [J].
HORIKAMI, SM ;
MOYER, SA .
JOURNAL OF VIROLOGY, 1991, 65 (10) :5342-5347
[13]   AN AMINO-PROXIMAL DOMAIN OF THE L-PROTEIN BINDS TO THE P-PROTEIN IN THE MEASLES-VIRUS RNA-POLYMERASE COMPLEX [J].
HORIKAMI, SM ;
SMALLWOOD, S ;
BANKAMP, B ;
MOYER, SA .
VIROLOGY, 1994, 205 (02) :540-545
[14]   VESICULAR STOMATITIS-VIRUS RNA REPLICATION - A ROLE FOR THE NS PROTEIN [J].
HOWARD, M ;
WERTZ, G .
JOURNAL OF GENERAL VIROLOGY, 1989, 70 :2683-2694
[15]   MEASLES-VIRUS PHOSPHOPROTEIN RETAINS THE NUCLEOCAPSID PROTEIN IN THE CYTOPLASM [J].
HUBER, M ;
CATTANEO, R ;
SPIELHOFER, P ;
ORVELL, C ;
NORRBY, E ;
MESSERLI, M ;
PERRIARD, JC ;
BILLETER, MA .
VIROLOGY, 1991, 185 (01) :299-308
[16]   SEPARATE DOMAINS OF SENDAI VIRUS-P PROTEIN ARE REQUIRED FOR BINDING TO VIRAL NUCLEOCAPSIDS [J].
RYAN, KW ;
PORTNER, A .
VIROLOGY, 1990, 174 (02) :515-521
[17]   2 NONCONTIGUOUS REGIONS OF SENDAI VIRUS P-PROTEIN COMBINE TO FORM A SINGLE NUCLEOCAPSID BINDING DOMAIN [J].
RYAN, KW ;
MORGAN, EM ;
PORTNER, A .
VIROLOGY, 1991, 180 (01) :126-134
[18]   DELETION ANALYSIS DEFINES A CARBOXYL-PROXIMAL REGION OF SENDAI VIRUS P-PROTEIN THAT BINDS TO THE POLYMERASE L-PROTEIN [J].
SMALLWOOD, S ;
RYAN, KW ;
MOYER, SA .
VIROLOGY, 1994, 202 (01) :154-163
[19]   MAPPING OF INTERACTING DOMAINS BETWEEN THE NUCLEOCAPSID PROTEIN AND THE PHOSPHOPROTEIN OF VESICULAR STOMATITIS-VIRUS BY USING A 2-HYBRID SYSTEM [J].
TAKACS, AM ;
DAS, T ;
BANERJEE, AK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (21) :10375-10379
[20]   CHARACTERIZATION OF V-PROTEIN IN MEASLES VIRUS-INFECTED CELLS [J].
WARDROP, EA ;
BRIEDIS, DJ .
JOURNAL OF VIROLOGY, 1991, 65 (07) :3421-3428