MUTAGENIC ACTIVITY OF ANTI-CANCER AGENT CIS-DICHLORODIAMMINE PLATINUM-II

被引：47

作者：

WIENCKE, JK ^{[1
]}

CERVENKA, J ^{[1
]}

PAULUS, H ^{[1
]}

机构：

[1] UNIV MINNESOTA,SCH DENT,DIV ORAL PATHOL & HUMAN & ORAL GENET,MINNEAPOLIS,MN 55455

来源：

MUTATION RESEARCH | 1979年 / 68卷 / 01期

关键词：

D O I：

10.1016/0165-1218(79)90079-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

cis-Dichlorodiamminoplatinum-II (cis-DDP) has been widely used as an anticancer chemotherapeutic agent. The mutagenicity of (cis-DDP) was investigated in vitro and in vivo using sister-chromatid exchange analysis and the analysis of chromosomal aberrations. Parallel human lymphocyte cultures were incubated with and without the addition of BrdU at 4 concentrations of cis-DDP. Significant increases in SCE rate were observed at 0.25 μg/ml and higher, showing a clear dose-response relation between SCE rate and cis-DDP concentration. A significant increase in chromosome breakage and tetraradial figures was observed in BrdU free cultures treated with cis-DDP again showing a dose dependency. Analysis of the distribution of cells in the first, second and third division in cis-DDP treated cultures demonstrated the depressing effect of the drug on mitotic activity. In vivo analysis of SCE and chromosome aberrations in mouse showed that 13.85 mg/kg i.p. of cis-DDP produces significant increases in the rate of SCE and chromosome aberrations in bone-marrow cells. © 1979.

引用

页码：69 / 77

页数：9

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