PEROXISOMAL-MICROSOMAL COMMUNICATION IN UNSATURATED FATTY-ACID METABOLISM

被引：18

作者：

BAYKOUSHEVA, SP ^{[1
]}

LUTHRIA, DL ^{[1
]}

SPRECHER, H ^{[1
]}

机构：

[1] OHIO STATE UNIV,COLL MED,DEPT MED BIOCHEM,COLUMBUS,OH 43210

来源：

FEBS LETTERS | 1995年 / 367卷 / 02期

关键词：

MICROSOME; PEROXISOME; ARACHIDONIC ACID; 7,10,13,16-DOCOSATETRAENOIC ACID; BETA-OXIDATION; ACYLATION;

D O I：

10.1016/0014-5793(95)00565-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by beta-oxidation of [1-C-14]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity was due to microsomal contamination. The production of acid-soluble radioactivity from [1-C-14]7,10,13,16-22:4, but not from [3-C-14]7,10,13,16- 22:4 was independent of 1-acyl-GPC, with and without microsomes. By comparing rates of peroxisomal beta-oxidation with those for microsomal acylation, it was shown that the preferred metabolic fate of arachidonate, when added directly to incubations, or generated via beta-oxidation, was esterification by microsomal 1-acyl-GPC acyltransferase, rather than continued peroxisomal beta-oxidation.

引用

页码：198 / 200

页数：3

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