THE PRIMARY STRUCTURE OF AN ENTAMOEBA-HISTOLYTICA BETA-HEXOSAMINIDASE-A SUBUNIT

被引:11
作者
BEANAN, MJ [1 ]
BAILEY, GB [1 ]
机构
[1] MOREHOUSE SCH MED,DEPT MICROBIOL & IMMUNOL,ATLANTA,GA 30310
关键词
DNA SEQUENCE; PROTEIN SEQUENCE;
D O I
10.1111/j.1550-7408.1995.tb05919.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An Entamoeba histolytica gene (hex-AI) that encodes subunit A of the lysosomal enzyme beta-hexosaminidase has been cloned and sequenced. The inferred 59 kDa hex-A1 protein has the same molecular weight and 32% amino acid residue identity with the human and mouse proteins and 28% residue identity with the Dictyostelium protein. Northern blot analysis identified a mRNA of approximately 1.6 kb, which is in agreement with the expected size of a mRNA encoding the 522 amino acid hex-A1 protein. Southern blot analysis indicated the presence of at least two beta-hexosaminidase A subunit genes.
引用
收藏
页码:632 / 636
页数:5
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