DETERMINATION OF GLYCEROL IN WINE BY AMPEROMETRIC FLOW-INJECTION ANALYSIS WITH AN IMMOBILIZED GLYCEROL DEHYDROGENASE REACTOR

A glycerol sensor, in which glycerol dehydrogenase was immobilized on Amino-Cellulofine, was developed for the continuous determination of glycerol in wine. NADH produced by the enzyme reaction was monitored amperometrically with a platinum electrode. A sandwich method was used for sample injection as an effective mixing mode of NAD and sample. A typical calibration curve showed a slightly convex curve between response and glycerol concentration (0.1-2 mM; 1.8 x 10(-8)-3.6 x 10(-7) mol). The relative standard deviation for ten measurements of 9 x 10(-8) mol glycerol was 0.5%. The proposed method was used for wine analysis and was compared with an F-kit (enzymatic, spectrophotometric) method.