SCANNING TUNNELING MICROSCOPY OF A LIQUID-CRYSTALLINE PHASE OF POLY((DA-DT).(DA-DT)) INDUCED BY A HISTONE H-1 PEPTIDE

被引：11

作者：

CORATGER, R ^{[1
]}

CHAHBOUN, A ^{[1
]}

AJUSTRON, F ^{[1
]}

BEAUVILLAIN, J ^{[1
]}

ERARD, M ^{[1
]}

AMALRIC, F ^{[1
]}

机构：

[1] CNRS,CRBGC,F-31062 TOULOUSE,FRANCE

来源：

ULTRAMICROSCOPY | 1990年 / 34卷 / 03期

关键词：

D O I：

10.1016/0304-3991(90)90068-W

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

In this report, we present the first observations of uncoated poly((dA-dT).(dA-dT)) molecules organized in a liquid crystalline phase induced by the binding of a histone H-1 peptide. The effect of the peptide on the polymer condensation is clearly illustrated on the large-scale STM images which reveal a well defined spacing between parallel DNA helices. High resolution images of rare isolated molecules of poly((dA-dT).(dA-dT)) exhibit two sets of helical pitch values, 6 and 7.5 nm. While the lower value can be correlated with the pitch of poly((dA-dT).(dA-dT)), the larger one may arise from peptide binding in the polymer minor groove.

引用

页码：141 / 147

页数：7