NEW SHUTTLE VECTOR FOR CLONING IN BACILLUS-STEAROTHERMOPHILUS

被引：23

作者：

DEROSSI, E

BRIGIDI, P

WELKER, NE

RICCARDI, G

MATTEUZZI, D

机构：

[1] UNIV BOLOGNA,INTERDEPARTMENTAL CTR BIOTECHNOL,DEPT PHARMACEUT SCI,I-40126 BOLOGNA,ITALY

[2] UNIV PAVIA,DEPT GENET & MICROBIOL A BUZZATI TRAVERSO,I-27100 PAVIA,ITALY

[3] NORTHWESTERN UNIV,DEPT BIOCHEM MOLEC BIOL & CELL BIOL,EVANSTON,IL 60208

来源：

RESEARCH IN MICROBIOLOGY | 1994年 / 145卷 / 08期

关键词：

CLONING; PLASMID; BACILLUS STEAROTHERMOPHILUS; SHUTTLE VECTOR; PRP9;

D O I：

10.1016/0923-2508(94)90074-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Cloning vector plasmid pRP9 was constructed on the basis of the broad host-range plasmid pLM6. pRP9 was a small plasmid (2.9 kb), possessed a convenient polyrestriction site sequence and efficiently transformed Bacillus subtilis, Bacillus stearothermophilus and Escherichia coli. Furthermore, pRP9 presented a very high segregational stability in Bacillus hosts. Also, the structural stability in Bacillus strains, grown under selective pressure, of pRP9 carrying a 3-kb fragment, was high. No single-stranded and high-molecular weight pRP9 DNA was found in B. stearothermophilus. The host/vector systems described possessed all the properties required far efficient gene cloning.

引用

页码：579 / 583

页数：5

共 11 条

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[2]

BRON S, 1990, MOL BIOL METHODS BAC, P75

[3] PLASMID SCREENING IN THERMOPHILIC BACILLUS - PHYSICAL CHARACTERIZATION AND MOLECULAR-CLONING [J].