URINARY RESPONSE TO INVIVO LIPID-PEROXIDATION INDUCED BY VITAMIN-E-DEFICIENCY

被引：37

作者：

LEE, HS ^{[1
]}

SHOEMAN, DW ^{[1
]}

CSALLANY, AS ^{[1
]}

机构：

[1] UNIV MINNESOTA,DEPT FOOD SCI & NUTR,ST PAUL,MN 55108

来源：

LIPIDS | 1992年 / 27卷 / 02期

关键词：

D O I：

10.1007/BF02535811

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Experiments were carried out to measure the urinary excretion of free and conjugated malonaldehyde (MDA) and other thiobarbituric acid reactive substances (TBARS) in vitamin E deficient and vitamin E supplemented rats. From both dietary groups, six TBA positive fractions were isolated, in addition to that containing free MDA, by high-performance liquid chromatography (HPLC) on a TSK-GEL G-1000PW column. Three of the fractions isolated were found to be significantly increased in vitamin E deficiency. After acid hydrolysis, only one of the above compounds produced free MDA which indicated the presence of derivatized MDA. Only this fraction exhibited fluorescence at excitation 370 nm and emission 450 nm. The five other fractions formed 2,4-dinitrophenylhydrazones (2,4-DNPH), indicating the presence of carbonyl groups, but the derivatized MDA fraction did not. No significant differences were found in free MDA levels between the vitamin E deficient and the vitamin E supplemented groups.

引用

页码：124 / 128

页数：5

共 34 条

[1] FLUORESCENT PRODUCTS OF PHOSPHOLIPIDS DURING LIPID PEROXIDATION [J].