RECOMBINANT BASIC FIBROBLAST GROWTH-FACTOR IN RED-BLOOD-CELL GHOSTS ACCELERATES INCISIONAL WOUND-HEALING

被引:37
作者
SLAVIN, J
HUNT, JA
NASH, JR
WILLIAMS, DF
KINGSNORTH, AN
机构
[1] UNIV LIVERPOOL, DEPT SURG, POB 147, LIVERPOOL L69 3BX, ENGLAND
[2] UNIV LIVERPOOL, DEPT PATHOL, LIVERPOOL L69 3BX, ENGLAND
[3] UNIV LIVERPOOL, INST CLIN BIOENGN, LIVERPOOL L69 3BX, ENGLAND
关键词
D O I
10.1002/bjs.1800790921
中图分类号
R61 [外科手术学];
学科分类号
摘要
The pharmacological manipulation of wound healing with locally applied growth factors is now a practical possibility. The effect of topical applications of recombinant basic fibroblast growth factor (bFGF) on the strength and cellularity of healing incisional rat skin wounds was investigated. Applications of bFGF in a simple vector (either a collagen suspension or saline) were not associated with any positive effects on wound breaking load at 7 days after injury in comparison with vector-treated control wounds; at the highest dose of 50 mug per wound, breaking loads were significantly decreased from a mean (s.e.m.) of 287(22) g/cm2 in controls to 201(23) g/cm2 (P < 0.005). Increasing doses of applied peptide were paralleled by increasing wound cellularity. Delay of bFGF release at the site of application was achieved by encapsulation into red blood cell ghosts. Wounds treated with bFGF in such ghosts were 50 per cent stronger than paired control wounds (388(27) versus 256(28) g/cm2, p < 0.002) 7 days after injury. Treated wounds were significantly more cellular at 4 days than paired control wounds. Topical applications of bFGF applied at the time of injury exert a positive effect on incisional wound strength only when a vector that delays release is used.
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页码:918 / 921
页数:4
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